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Plant Cell Morphogenesis Viktor Zarsky

Plant Cell Morphogenesis By Viktor Zarsky

Plant Cell Morphogenesis by Viktor Zarsky


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Summary

This book features cutting-edge methods and protocols in plant cell morphogenesis in the MiMB standard format. It provides step-by-step detail essential for reproducible results and contains expert implementation advice.

Plant Cell Morphogenesis Summary

Plant Cell Morphogenesis: Methods and Protocols by Viktor Zarsky

Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on classical light microscopy and histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized electron microscopy techniques, and methods used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by expert researchers in the field and include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Plant Cell Morphogenesis Reviews

From the book reviews:

A compendium of procedures and techniques for investigating the structure and shape of plant tissues and cells in a developmental context. ... I can certainly rate this book very useful for the scientific community, as it contains many valuable new techniques and inside knowledge. Furthermore, it could inspire researchers to use new technical approaches for their research topics and discuss these with the expert authors of these articles. (Kai R. Konrad, Journal of Plant Physiology, Vol. 171, 2014)

Table of Contents

1. Essential Methods of Plant Sample Preparation for Light Microscopy

Ales Soukup and Edita Tylova

2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy

Ales Soukup

3. Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues

Kieran J. D. Lee and J. Paul Knox

4. Automated Microscopy in Forward Genetic Screening of Arabidopsis

Tereza Dobisova and Jan Hejatko

5. Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections

Jana Albrechtova, Zuzana Kubinova, Ales Soukup, and Jiri Janacek

6. Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells

Andreas Nebenfuhr

7. Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM

Amparo Rosero, Viktor Zarsky, and Fatima Cvrckova

8. Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces

Dorota Kwiatkowska and Agata Burian

9. Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope

Olivier Hamant, Pradeep Das, and Agata Burian

10. Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX

Pierre Barbier de Reuille, Sarah Robinson, and Richard S. Smith

11. Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy

Anne-Lise Routier-Kierzkowska and Richard S. Smith

12. High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy

Ichirou Karahara and Byung-Ho Kang

13. Reconstructing Plant Cells in 3D by Serial Section Electron Tomography

Kiminori Toyooka and Byung-Ho Kang

14. Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy

Jindriska Fiserova and Martin W. Goldberg

15. Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections

Ilse Foissner and Margit Hoeftberger

16. Live Cell Imaging of Arabidopsis Root Hairs

Tijs Ketelaar

17. Morphological Analysis of Cell Growth Mutants in Physcomitrella

Luis Vidali and Jeffrey P. Bibeau

18. Plant Cell Lines in Cell Morphogenesis Research

Daniela Seifertova, Petr Klima, Marketa Parezova, Jan Petrasek, Eva Zazimalova, and Zdenek Opatrny

19. Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes

Radek Bezvoda, Roman Pleskot, Viktor Zarsky, and Martin Potocky

20. Lab-on-a-Chip for Studying Growing Pollen Tubes

Carlos G. Agudelo, Muthukumaran Packirisamy, and Anja Geitmann

21. Laser Microdissection of Plant Cells

Yvonne Ludwig and Frank Hochholdinger

22. Optical Trapping in Plant Cells

Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren

23. Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery

Fatima Cvrckova and Michal Hala

Additional information

NLS9781493960323
9781493960323
1493960326
Plant Cell Morphogenesis: Methods and Protocols by Viktor Zarsky
New
Paperback
Humana Press Inc.
2016-08-23
285
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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