Series Preface...
Preface...
Table of Contents...
Contributing Authors...
Part I Molecular Techniques
1. Production of High-Quality Antibodies for the Study of Receptors and Ion Channels
Masahiko Watanabe
2. Co-Immunoprecipitation from Brain
Xavier Morato, Dasiel O. Borroto-Escuela, Kjell Fuxe, Victor Fernandez-Duenas, and Francisco Ciruela
3. Sub-Synaptic Membrane Fractionation
Paula M. Canas and Rodrigo A. Cunha
4. Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation
Elek Molnar
5. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells
Juan Varela, Julien Dupuis, Francois Maingret, and Laurent Groc
6. Radioligand Binding Detection of Receptors in Brain Membranes
Fuencisla Pilar-Cuellar, Emilio Garro-Martinez, Elena Castro, and Alvaro Diaz
7. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain
Markus U. Ehrengruber and Kenneth Lundstrom
8. Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain
Victor Fernandez-Duenas, Thierry Durroux, and Francisco Ciruela
9. Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay
Manuel Narvaez, Minerva Crespo-Ramirez, Ramon Fores-Pons, Mariana Pita-Rodriguez, Francisco Ciruela, Malgorzata Filip, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Miguel Perezde la Mora, Kjell Fuxe, and Dasiel O. Borroto-Escuela
10. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain
Marta Valle-Leon, Victor Fernandez-Duenas, and Francisco Ciruela
Part II Neuroanatomical Techniques
11. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling
Miwako Yamasaki and Masahiko Watanabe
12. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain
Rebeca Vidal, Fuencisla Pilar-Cuellar, Alvaro Diaz, and Elena Castro
13. Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting
Elek Molnar
14. Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility
Kohtarou Konno and Masahiko Watanabe
15. Localization of GFP-Tagged Proteins at the Electron Microscope
Sara Gil-Perotin, A. Cebrian-Silla, V. Herranz-Perez, P. Garcia-Belda, S. Gil-Garcia, M. Fil, J.S. Lee, M.V. Nachury, and Jose Manuel Garcia-Verdugo
16. Tyramide Signal Amplificacion for Immunoelectron Microscopy
Maria Jose Ulloa-Navas, Patricia Garcia-Tarraga, Susana Gonzalez-Granero, Pedro Perez-Borreda, Vicente Herranz-Perez, and Jose Manuel Garcia-Verdugo
17. Pre-Embedding Methods for the Localization of Receptors and Ion Channels
Rafael Lujan
18. Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels
Rafael Lujan and Masahiko Watanabe
19. High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)
Walter A. Kaufmann, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto
20. Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM
Marta Turegano-Lopez, Jose Rodrigo-Rodriguez, Lidia Alonso-Nanclares, Juncal Gonzalez-Soriano, Javier DeFelipe, and Angel Merchan-Perez
21. Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies
Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta
22. Efficient Labeling of Neurons and Identification of Postsynaptic Sites Using Adeno-Associated Virus Vector
Megumu Takahashi, Yoko Ishida, Naoya Kataoka, Kazuhiro Nakamura, and Hiroyuki Hioki
23. Analysis of Synaptic Connections at the Electron Microscopic Level using Sindbis Virus Vectors
Takahiro Furuta, Keiko Okamoto-Furuta, and Hiroyuki Hioki
24. Morphological and Neurochemical Characterization of Electrophysiologically Identified Cells
Yoshiyuki Kubota
Part III Functional Techniques
25. Using Electrophysiology to Study Synaptic and Extrasynaptic Ionotropic Receptors in Hippocampal Neurons
Ian D. Coombs and David Soto
26. Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels
Norbert Weiss and Michel De Waard
27. Electrophysiological Recordings in Behaving Animals
Agnes Gruart and Jose M. Delgado-Garcia
28. Voltammetry in Behaving Animals
Kendra D. Bunner and George V. Rebec
29. In Vivo Brain Microdialysis of Monoamines
Jorge E. Ortega, Blanca Perez-Palomar, J. Javier Meana, and Luis F. Callado
30. Optical Control of Brain Receptors using Photoactive Drug in Behaving Animals
Marc Lopez-Cano, Victor Fernandez-Duenas, and Francisco Ciruela
31. Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor
Victor Fernandez-Duenas, Xavier Morato, Thomas Knoepfel, and Francisco Ciruela
Chapter 32. Monitoring GPCR-Mediated cAMP Accumulation in Rat Striatal Synaptosomes
Jaume Taura, Victor Fernandez-Duenas, and Francisco Ciruela
33. GPCR-Mediated MAPK/ERK Cascade Activation in Mouse Striatal Slices
Maricel Gomez-Soler, Victor Fernandez-Duenas, and Francisco Ciruela
Subject Index List...