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Flower Development Jose Luis Riechmann

Flower Development By Jose Luis Riechmann

Flower Development by Jose Luis Riechmann


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Summary

In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches.

Flower Development Summary

Flower Development: Methods and Protocols by Jose Luis Riechmann

In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls

Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.

Table of Contents

Part I: Review and Overview Chapters

1. Flower Development in Arabidopsis - There is More to it than Learning your ABCs

Nathanael Prunet and Thomas Jack

2. Flower Development in the Asterid Lineage

Barry Causier and Brendan Davies

3. Grass Flower Development

Hiro-Yuki Hirano, Wakana Tanaka, and Taiyo Toriba

4. Flower Diversity and Angiosperm Diversification

Pamela S. Soltis and Douglas E. Soltis

5. Flower Development - Open Questions and Future Directions

Frank Wellmer, John L. Bowman, Brendan Davies, Cristina Ferrandiz, Jennifer C. Fletcher, Robert G. Franks, Emmanuelle Graciet, Veronica Gregis, Toshiro Ito, Thomas P. Jack, Yuling Jiao, Martin M. Kater, Hong Ma, Elliot M. Meyerowitz, Nathanael Prunet, Jose Luis Riechmann

PART II: Genetic and Phenotypic Analyses

6. Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors

Thanh Theresa Dinh, Elizabeth Luscher, Shaofang Li, Xigang Liu, So Youn Won, and Xuemei Chen

7. Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development

Mona M. Monfared and Jennifer C. Fletcher

8. Genetic and Phenotypic Analyses of Petal Development in Arabidopsis

Judit Szecsi, Barbara Wippermann and Mohammed Bendahmane

9. Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsisand Rice

Fang Chang, Zaibao Zhang, Yue Jin, and Hong Ma

10. Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana

Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma

11. Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis

Vicente Balanza, Patricia Ballester, Monica Colombo, Chloe Fourquin, Irene Martinez-Fernandez, and Cristina Ferrandiz

Part III: Microscopy and Histology

12. Microscopic Analysis of Arabidopsis Ovules

Balaji Enugutti and Kay Schneitz

13. Scanning Electron Microscopy Analysis of Floral Development

Robert G. Franks

14. Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue

Anna Medzihradszky, Kay Schneitz, and Jan U. Lohmann

15. The GUS Reporter System in Flower Development Studies

Janaki S. Mudunkothge and Beth A. Krizek

Part IV: Experimental Systems

16. A Floral Induction System for the Study of Early Arabidopsis Flower Development

Diarmuid O'Maoileidigh and Frank Wellmer

17. Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types

G. Venugopala Reddy

18. Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers

Ying Wang and Yuling Jiao

19. Laser-assisted Microdissection Applied to Floral Tissues

Samuel E. Wuest and Ueli Grossniklaus

PartV: Molecular Biology, Genomics, and Systems Biology

20. Identification of Arabidopsis Knock-out Lines for Genes of Interest

Jose Tomas Matus, Thilia Ferrier, and Jose Luis Riechmann

21. Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues

Mariana Bustamante, Jian Jin, Oriol Casagran, Tania Nolan, and Jose Luis Riechmann

22. Misexpression Approaches for the Manipulation of Flower Development

Yifeng Xu, Eng-Seng Gan, and Toshiro Ito

23. Next Generation Sequencing Applied to Flower Development: RNA-Seq

Jun He and Yuling Jiao

24. Next-Generation Sequencing Applied to Flower Development: ChIP-Seq

Emmanuelle Graciet, Diarmuid Seosamh O'Maoileidigh, and Frank Wellmer

25. Live-Imaging of the Arabidopsis Inflorescence MeristemMarcus G. Heisler and Carolyn Ohno

26. Gene Regulatory Network Models for Floral Organ Determination

Eugenio Azpeitia, Jose Davila-Velderrain, Carlos Villarreal, and Elena R. Alvarez-Buylla

Additional information

NLS9781493943401
9781493943401
1493943405
Flower Development: Methods and Protocols by Jose Luis Riechmann
New
Paperback
Humana Press Inc.
2016-08-27
475
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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