FOREWORD ix PREFACE xi CONTRIBUTORS xiii PART I ANTIBODY BASICS. 1. Therapeutic Monoclonal Antibodies: Past, Present, and Future 3 William R. Strohl 2. Antibody Molecular Structure 51 Robyn L. Stanfield and Ian A. Wilson 3. Glycosylation of Therapeutic IgGs 67 Yusuke Mimura, Roy Jefferis, Yuka Mimura-Kimura, Jodie Abrahams, and Pauline M. Rudd 4. Antibody Databases and Tools: The IMGTw Experience 91 Marie-Paule Lefranc PART II ANTIBODY SOURCES. 5. Human Antibodies from Transgenic Mice 117 Nils Lonberg 6. Rabbit Hybridoma 151 Weimin Zhu and Guo-Liang Yu 7. Human Antibody Repertoire Libraries 169 David Lowe and Tristan J. Vaughan PART III IN VITRO DISPLAY TECHNOLOGY. 8. Antibody Phage Display 191 Michael Hust, Holger Thie, Thomas Schirrmann, and Stefan Dubel 9. Yeast Surface Display 213 Jennifer L. Lahti and Jennifer R. Cochran 10. Ribosomal Display 239 George Thom 11. Bacterial Display of Antibodies 255 Thomas J. Van Blarcom and Barrett R. Harvey 12. Antibody Selection from Immunoglobulin Libraries Expressed in Mammalian Cells 283 Ernest S. Smith and Maurice Zauderer PART IV ANTIBODY ENGINEERING. 13. Antibody Engineering: Humanization, Affinity Maturation, and Selection Techniques 311 Juan C. Almagro and William R. Strohl 14. Modulation of Serum Protein Homeostasis and Transcytosis by the Neonatal Fc Receptor 335 William F. Dall'Acqua and Herren Wu 15. Engineering the Antibody Fc Region for Optimal Effector Function 349 Greg A. Lazar and John R. Desjarlais PART V PHYSIOLOGY AND IN VIVO BIOLOGY. 16. Antibody-Complement Interaction 373 Kileen L. Mershon and Sherie L. Morrison 17. Bacteria Immunoglobulin-Binding Proteins: Biology and Practical Applications 385 Leslie Cope and Tessie McNeely 18. Immunogenicity Screening Using in Silico Methods: Correlation between T-Cell Epitope Content and Clinical Immunogenicity of Monoclonal Antibodies 417 Si-Han Hai, Julie A. McMurry, Paul M. Knopf, William Martin, and Anne S. De Groot 19. Monoclonal Antibody Pharmacokinetics and Pharmacodynamics 439 Christopher R. Gibson, Punam Sandhu, and William D. Hanley 20. Biodistribution and Imaging 461 Tove Olafsen and Anna M. Wu 21. Antibodies and the Blood-Brain Barrier 483 Angela R. Jones and Eric V. Shusta PART VI ANTIBODY CHARACTERIZATION. 22. Determination of Equilibrium Dissociation Constants 505 Robin E. Ernst, Katrina N. High, Tom R. Glass, and Qinjian Zhao 23. Molecular and Functional Characterization of Monoclonal Antibodies 525 Qinjian Zhao, Terrance A. Stadheim, Lorenzo Chen, and Michael W. Washabaugh 24. Characterization of Heterogeneity in Monoclonal Antibody Products 541 Yang Wang, Michael W. Washabaugh, and Qinjian Zhao PART VII ANTIBODY EXPRESSION. 25. Antibody Expression in Mammalian Cells 557 Fubao Wang, Lorenzo Chen, Neal Connors, and Henryk Mach 26. Production of Antibodies in Pichia pastoris 573 Juergen H. Nett 27. Production of Antibody Fab0 Fragments in E. coli 589 David P. Humphreys and Leigh Bowering 28. Production of Human Therapeutic Monoclonal Antibodies in Chicken Eggs 623 Lei Zhu and Robert J. Etches 29. Production of Antibodies in Plants 651 Kevin M. Cox, Jeffrey T. Regan, Jason D. Sterling, Vincent P. M. Wingate, and Lynn F. Dickey PART VIII THERAPEUTIC ANTIBODIES. 30. The Formulation and Delivery of Monoclonal Antibodies 675 Vikas K. Sharma, Hung-Wei Chih, Randall J. Mrsny, and Ann L. Daugherty 31. Therapeutic Antibodies in Clinical Use and Leading Clinical Candidates 711 Ningyan Zhang, Brent R. Williams, Ping Lu, Zhiqiang An, and Chen-Ni Chin 32. Follow-On Protein Products: What, Where, When, How? 763 Brent R. Williams and William R. Strohl 33. Monomeric Fc Fusion Molecules 779 Jennifer A. Dumont, Susan C. Low, Robert T. Peters, and Alan J. Bitonti 34. Radioimmunotherapy: Current Status and Future Directions 797 Neeta Pandit-Taskar and Chaitanya R. Divgi 35. Antibody-Drug Conjugate Therapy 821 Stephen C. Alley, Dennis Benjamin, and Che-Leung Law ABBREVIATIONS 857 INDEX 865
