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Plant Metabolic Engineering Vladimir Shulaev

Plant Metabolic Engineering By Vladimir Shulaev

Plant Metabolic Engineering by Vladimir Shulaev


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Summary

This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering.

Plant Metabolic Engineering Summary

Plant Metabolic Engineering: Methods and Protocols by Vladimir Shulaev

This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant and microbial systems; and omics technologies, such as transcriptomics, proteomics, metabolomics, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Metabolic Engineering: Methods and Protocols is a valuable resource for biologists, chemists, biotechnologists, students, and broad cohorts of researchers who works in the fields of plant metabolism and metabolic engineering.


Table of Contents

Preface...Table of Contents...Contributing Authors...
1. Methods for the Development of Recombinant Microorganisms for the Production of Natural Products Alexander Perl, YeJong Yoo, Hunter Dalton, and Mattheos A. G. Koffas
2. Sustainable Technological Methods for the Extraction of Phytochemicals from Citrus ByproductsSalvatore Multari, Fulvio Mattivi, and Stefan Martens
3. Reconstitution of Metabolic Pathway in Nicotiana benthamianaChenggang Liu
4. A Protocol for Phylogenetic Reconstruction Soham Sengupta and Rajeev K. Azad
5. RNA-Seq Data Analysis Pipeline for Plants: Transcriptome Assembly, Alignment, and Differential Expression AnalysisDavid J. Burks and Rajeev K. Azad
6. A Protocol for Horizontally Acquired Metabolic Gene Detection in AlgaeRavi S. Pandey and Rajeev K. Azad
7. Global Comparative Label-Free Yeast Proteome Analysis by LC-MS/MS After High-pH Reversed-Phase Peptide Fractionation Using Solid-Phase Extraction CartridgesKhadiza Zaman, Prajita Pandey, Vladimir Shulaev, and Laszlo Prokai
8. Gas Chromatography Coupled to Atmospheric Pressure Chemical IonizationHigh Resolution Mass Spectrometry for Metabolite Fingerprinting of Grape (Vitis vinifera L.) BerryJohana S. Revel, Armando Alcazar Magana, Jeffrey Morre, Laurent Deluc, and Claudia S. Maier
9. GC-MS/MS Profiling of Plant MetabolitesFeroza Kaneez Choudhury, Prajita Pandey, Ron Mittler, Dwain Cardona, Amit C. Gujar, and Vladimir Shulaev
10. Analysis of Grape Volatiles using Atmospheric Pressure Ionization Gas Chromatography Mass Spectrometry-Based MetabolomicsManoj Ghaste, Fulvio Mattivi, Giuseppe Astarita, and Vladimir Shulaev
11. A High-Throughput HILIC-MS-Based Metabolomic Assay for the Analysis of Polar Metabolites Giuseppe Paglia and Giuseppe Astarita
12. Macrolipidomic Profiling of Vegetable Oils: The Analysis of Sunflower Oils with Different Oleic Acid ContentJuan J. Aristizabal-Henao and Ken D. Stark
13. Non-Targeted Lipidomics using a Robust and Reproducible Lipid Separation using UPLC with Charged Surface Hybrid Technology and High Resolution Mass SpectrometryGiorgis Isaac, Vladimir Shulaev, and Robert S. Plumb
14. Comprehensive Analysis of Plant Lipids using Sub-2m Particle CO2-Based Chromatography Coupled to Mass SpectrometryCarolina Salazar, Michael D. Jones, Giorgis Isaac, and Vladimir Shulaev
15. Bioinformatics in Lipidomics: Automating Large-Scale LC-MS-Based Untargeted Lipidomics Profiling with SimLipid SoftwareNingombam Sanjib Meitei and Vladimir Shulaev
16. A Protocol for Prion Discovery in Plants Jamie D. Dixson and Rajeev K. Azad
17. Structural Determination of Uridine Diphosphate Glycosyltransferases using X-Ray CrystallographyKasandra Alderete and Xiaoqiang Wang
Subject Index List...

Additional information

NLS9781071618240
9781071618240
1071618245
Plant Metabolic Engineering: Methods and Protocols by Vladimir Shulaev
New
Paperback
Springer-Verlag New York Inc.
2022-11-18
244
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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