Retinal Development: Methods and Protocols by Shu-Zhen Wang

In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels. These advanced methodologies have allowed and will continue to allow researchers to gain new insights into the molecular mechanisms underlying retinal development. In Retinal Development: Methods and Protocols, expert researchers in the field detail many of the protocols used for a wide range of experiments. These include protocols and techniques for manipulating gene expression in vivo, tracing cell fates with modernized classic blastomere manipulation in Xenopus and with Cre-based technique in mouse and in zebrafish, retinal regeneration and stem cell-based replacement, and ERG (function) recording and non-invasive imaging. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Retinal Development: Methods and Protocols provides methodologies crucial to the success of increasingly more complex and often challenging investigations in the fields of retinal development and other biological and biomedical research.

^{Part I: Manipulation of Gene Expression in vivo}

^{1. Conditional Control of Gene Expression in the Mouse Retina}

^{Qian Ding and Lin Gan}

^{2. Generation Transgenic Xenopus using Restriction Enzyme-Mediated Intergration}

^{Mohammad Haeri and Barry E. Knox}

^{3. In vivo Functional Analysis of Transcription Factor--response Element Interaction using Transgenic Xenopus laevis}

^{Heithem M. El-Hodiri, Yi Pan, and Lisa E. Kelly}

^{4. Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse Eyes}

^{John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Johnson, Lennart Berglin, T. Michael Redmond, Jeffrey H. Boatright}

^{5. In Ovo Eye Electroporation}

^{Teri L. Belecky-Adams, Scott R. Hudson, and Sarika Tiwari}

^{6. Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in Xenopus Embryos}

^{Sally A. Moody}

^{Also see Chapter 8.}

^{Part II: Tracing Cell Fate}

^{7. Testing Retina Fate Commitment in Xenopus by Blastomere Deletion, Transplantation and Explant Culture}

^{Sally A. Moody}

^{8. Application of Cre-loxP Recombination for Lineage Tracing of Adult Zebrafish Retinal Stem Cells}

^{Rajesh Ramachandran, Aaron Reifler, Jin Wan, and Daniel Goldman}

^{9. Fate Tracing of neurogenin2-expressing Cells in the Mouse Retina using CreER^TM - LacZ}

^{Wenxin Ma and Shu-Zhen Wang}

^{Part III: In vitro Systems}

^{10. In Vitro Explant Culture and Related Protocols for the Study of Mouse Retinal Development}

^{Kangxin Jin and Mengqing Xiang}

^{11. In vitro Biochemical Assays to Monitor Rhodopsin Function}

^{Joshua Sammons and Alecia K. Gross}

^{12. Transfection of Primary Embryonic Chicken Retinal Cells using Cationic Lipid}

^{Yi-Wen Hsieh and Xian-Jie Yang}

^{13. Production of High Titer RCAS Retrovirus}

^{Run-Tao Yan and Shu-Zhen Wang}

^{14. Chick RPE Transdifferentiation Assay for Proneural Activities}

^{Shu-Zhen Wang and Run-Tao Yan}

^{Part IV: Regeneration/Stem Cells}

^{15. Studying the Generation of Regenerated Retinal Neuron from Muller glia in the Mouse Eye}

^{Mike O. Karl and Thomas A. Reh}

^{16. Production and Transplantation of Retinal Cells from Human and Mouse Embryonic Stem Cells}

^{Anna La Torre, Deepak A Lamba, Anu Jayabalu, Thomas A Reh}

^{17. Light-induced Photoreceptor Degeneration in the Retina of the Zebrafish}

^{Scott Taylor, Jing Chen, Jing Luo and Peter Hitchcock}

^{18. Microarray-based Gene Profiling Analysis of Muller Glia-derived Retinal Stem Cells in Light-damaged Retinas from Adult Zebrafish}

^{Zhao Qinand Pamela A. Raymond}

^{Also see Chapter 8.}

^{Part V: Function/Imaging}

^{19: Measuring Rodent Electroretinograms to Assess Retinal Function}

^{Molly E. Clark and Timothy W. Kraft}

^{20. Functional Imaging of Retinal Photoreceptors and Inner Neurons using Stimulus-evoked Intrinsic Optical Signals}

^{Xin-Cheng Yao and Yi-Chao Li}

^{Part VI: Emerging Methodologies}

^{21. Use of Laser Capture Microdissection for Analysis of Retinal mRNA/miRNA Expression and DNA Methylation}

^{Laszlo Hackler Jr, Tomohiro Masuda, Verity F. Oliver, Shannath L. Merbs, and Donald J.Zack}

^{^{22. Revealing Looping Organization of Mammalian Photoreceptor Genes Using Chromosome Conformation Capture (3C) Assays}}

^{^{Guang-Hua Peng and Shiming Chen}}

^{^{23. Retinal Transcriptome Profiling by Directional Next Generation Sequencing Using 100 ng of Total RNA}}

^{^{Matthew John Brooks, Harsha Karur Rajasimha, and Anand Swaroop}}

^{^{24. Exome Sequencing: Capture and Sequencing of All Human Coding Regions for Disease Gene Discovery}}

^{^{Rinki Ratna Priya, Harsha Karur Rajasimha, Matthew Brooks, and Anand Swaroop}}

^{^{25. Determination of Post-translational Modifications of Photoreceptor Differentiation Factor NRL: Focus on SUMOylation}}

^{^{Jerome E. Roger, Jacob Nellissery and Anand Swaroop}}

Retinal Development Shu-Zhen Wang

Retinal Development by Shu-Zhen Wang

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Retinal Development Summary

Retinal Development: Methods and Protocols by Shu-Zhen Wang

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