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T-Cell Development Remy Bosselut

T-Cell Development By Remy Bosselut

T-Cell Development by Remy Bosselut

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T-Cell Development Summary

T-Cell Development: Methods and Protocols by Remy Bosselut

This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology.Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses,Innate Lymphoid Cells (ILC), mouse T-cell development,antigen receptor-less cousins of T cells,bone marrow chimeras,thymic stroma,and multiple aspects of thymocyte biology.Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,T-Cell Development: Methods and Protocols, Second Edition aims to be auseful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.

Table of Contents

A beginners guide to T cell development.-Revelations in thymic epithelial cell biology and heterogeneity from single cell RNA sequencing and lineage tracing methodologies.-Early development of innate lymphoid cells.-Development of gd T cells, Soldiers on the Front Lines of Immune Battles.-Principles of Advanced Flow Cytometry: A Practical Guide.-Genetic strategies to study T cell development.-A simplified approach to evaluating T cell development by flow cytometry.-Purification of thymocyte and T cell subsets.-Generation of bone marrow chimeras.-Generation of murine T cell effector populations in vitro.-Large-scale isolation of mouse thymic epithelial cells.-Generation of retrogenic mice to investigate T cell developmentPurification of bone marrow precursors to T cells and ILCs.-Studying T cell Development in Neonatal and Adult Thymic Slices.-Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines.-Methods for Study of Mouse T Cell Receptor a and b Gene Rearrangements.-Ultrasound guided intra-thymic cell injection.-Fetal thymic organ culture and negative selection.-In Vitro Analysis of Thymocyte Signaling.-Identification and purification of human T cell precursors.-In vitro model systems to study human T cell development.-Zebrafish: A Tractable Model for Analysis of T cell Development.


Additional information

NPB9781071627426
9781071627426
1071627422
T-Cell Development: Methods and Protocols by Remy Bosselut
Remy Bosselut
Methods in Molecular Biology
New
Paperback
Springer-Verlag New York Inc.
2023-11-15
384
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
This is a new book - be the first to read this copy. With untouched pages and a perfect binding, your brand new copy is ready to be opened for the first time

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