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Chloroplast Research in Arabidopsis R. Paul Jarvis

Chloroplast Research in Arabidopsis By R. Paul Jarvis

Chloroplast Research in Arabidopsis by R. Paul Jarvis


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Summary

In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system.

Chloroplast Research in Arabidopsis Summary

Chloroplast Research in Arabidopsis: Methods and Protocols, Volume II by R. Paul Jarvis

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Table of Contents

Part I: Multiprotein Complexes and Protein-Protein Interactions

1. One- and Two-Dimensional Blue Native (BN) PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes

Shingo Kikuchi, Jocelyn Bedard, and Masato Nakai

2. Analysis of Thylakoid Protein Complexes by Two Dimensional Electrophoretic Systems

Sari Sirpioe, Marjaana Suorsa, and Eva-Mari Aro

3. Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification

Charles Andres, Birgit Agne, and Felix Kessler

4. Studying Interactions between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation (BiFC) and Foerster Resonance Energy Transfer (FRET)

Jodi Maple and Simon G. Moller

5. Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods

Joanna Tripp and Enrico Schleiff

Part II: Omics and Large-Scale Analyses

6. Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis

Diogo Ribeiro Demartini, Celia Regina Carlini, and Jay J. Thelen

7. Use of Transcriptomics to Analyse Chloroplast Processes in Arabidopsis

Tatjana Kleine and Dario Leister

8. Use of Non-Aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis

Peter Geigenberger, Axel Tiessen, and Joerg Meurer

9. Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis

Yan Lu, Linda J. Savage, and Robert L. Last

Part III: Proteomics and Suborganellar Fractionation

10. Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies

Daniel Salvi, Lucas Moyet, Daphne Seigneurin-Berny, Myriam Ferro, Jacques Joyard, and Norbert Rolland

11. Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis

Michael Hall, Yogesh Mishra, and Wolfgang P. Schroeder

12. Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies

Celine Besagni, Lucia Eugeni Piller, and Claire Brehelin

13. Preparation and Proteomic Analysis of Chloroplast Ribosomes

Kenichi Yamaguchi

14. The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting

Giulia Friso, Paul Dominic B. Olinares, and Klaas J. van Wijk

15. Use of Phosphoproteomics to Study Post-Translational Protein Modifications in Arabidopsis Chloroplasts

Anne Endler and Sacha Baginsky

Part IV: Photosynthesis and Biochemical Analysis

16. Use of a Pulse-Amplitude Modulated (PAM) Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis in Arabidopsis Plants

Matthew D. Brooks and Krishna K. Niyogi

17. Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves

Giles Johnson and Erik Murchie

18. Measurement of the pH and Electric Field Developed Across Arabidopsis Thylakoids in the Light

Steven M. Theg and Curtis Tom

19. Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis

Aleel K. Grennan and Donald R. Ort

20. Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis

Olaf Czarnecki, Enrico Peter, and Bernhard Grimm

21. Analysis of Starch Metabolism in Chloroplasts

Carmen Hostettler, Katharina Koelling, Diana Santelia, Sebastian Streb, Oliver Koetting, and Samuel C. Zeeman

22. The Analysis of Lipid Content and Quality in Arabidopsis Plastids

Anna Maria Zbierzak, Peter Doermann, and Georg Hoelzl

Additional information

NLS9781493962891
9781493962891
1493962892
Chloroplast Research in Arabidopsis: Methods and Protocols, Volume II by R. Paul Jarvis
New
Paperback
Humana Press Inc.
2016-08-23
432
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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