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Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies Monica V. Cunha

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies By Monica V. Cunha

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies by Monica V. Cunha

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Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies Summary

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies by Monica V. Cunha

This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies Reviews

From the book reviews:

This is an overview of molecular biology techniques for the detection of pathological states in animals. ... The audience includes doctors, laboratory technicians, researchers, and students of veterinary medicine and science. ... This is a nicely written, clinically relevant, cutting-edge book that gives a thorough overview of numerous molecular biology techniques for the detection of pathological diseases. (Maureen McMichael, Doody's Book Reviews, March, 2015)

Table of Contents

PART I- Introducing Nucleic Acid Testing In The Veterinary Laboratory

1. Overview And Challenges Of Molecular Technologies In The Veterinary Microbiology Laboratory

Monica V. Cunha And Joao Inacio

2. Significance And Integration Of Molecular Diagnostics in the Framework of Veterinary Practice

Alicia Aranaz

3. Biosafety Principles And Practices for the Veterinary Diagnostic Laboratory

Joseph Kozlovac And Beverly Schmitt

4. Veterinary Biobank Facility: Development And Management For Diagnostic and Research Purposes

Tina Lombardo, Silvia Dotti, Riccardo Villa, Stefano Cinotti, And Maura Ferrari

5. Biological Specimen Collection And Processing for Molecular Analysis

Susan Sanchez And Holly Sellers

6. Validation Of Molecular Diagnostic Assays And Quality Assurance and Control in the Veterinary Laboratory

Nick Saunders and Ian Sharp

PART II - Molecular Detection and Identification of Animal Pathogens in Laboratorial Settings

7. Molecular Approaches To Recognize Relevant And Emerging Infectious Diseases In Animals

Fredrik Granberg, Oskar Karlsson, Mikael Leijon, Lihong Liu, and Sandor Belak

8. Real Time Reverse Transcriptase PCR For The Detection Of Bluetongue Virus

Carrie Batten, Lorraine Frost, And Chris Oura

9. Nested And Multiplex Real Time PCR Using Dual-Labeled Probes: Detecting And Discriminating Mycobacterium Tuberculosis Complex Members In Cultures And Animal Tissues

Pedro Costa, Isabel Couto, Miguel Viveiros, and Joao Inacio

10. A Real-Time PCR Assay For The Diagnosis Of Gastrointestinal Nematode Infections of Small Ruminants

Florian Roeber, Aaron R. Jex, And Robin B. Gasser

11. Improved Detection Of Mycobacterium Bovis In Bovine Tissues Using Immunomagnetic Separation Approaches

Irene R. Grant And Linda D. Stewart

12. Detection Of Fish Pathogens By Loop-Mediated Isothermal Amplification (LAMP) Technique

Hatem Soliman, Mona Saleh, And Mansour El-Matbouli

13. Direct Detection Of Theileria Annulata In Bovine Blood Samples Using Standard And Isothermal DNA Amplification Approaches

Jacinto Gomes And Joao Inacio

14. Reverse Line Blot Hybridization With Species-Specific Oligonucleotide Probes: Application To Piroplasms Detection

Ana Hurtado

15. DNA Microarray-Based Detection Of Multiple Pathogens: Mycoplasma Spp. and Chlamydia Spp.

Christiane Schnee and Konrad Sachse

16. In Situ Hybridization With Labeled Probes: Assessment of African Swine Fever Virus In Formalin-Fixed Paraffin-Embedded Tissues

Maria Ballester and Fernando Rodriguez

17. Fluorescence In Situ Hybridization For The Tissue Detection of Bacterial Pathogens Associated With Porcine Infections

Henrik Elvang Jensen, Louise Kruse Jensen, Kristiane Barington, Susanne Pors, Thomas Bjarnsholt, and Mette Boye

18. Identification Of Animal Pasteurellaceae By MALDI-TOF Mass Spectrometry

Joachim Frey and Peter Kuhnert

19. Gold Nanoparticles as a Potential Tool For Diagnosis of Fish Diseases

Mona Saleh, Hatem Soliman, and Mansour El-Matbouli

20. Nucleic Acid Testing, New Platforms And Nanotechnology For Point-of-Decision Diagnosis Of Animal Pathogens

Fernando Teles And Luis Pina Fonseca

PART III - Molecular Profiling Of Veterinary Relevant Microbial Pathogens

21. Molecular Typing Tools: From Pattern Recognition To Genome-Based Algorithms

Konrad Sachse and Petra Moebius

22. Analysis Of Campylobacter Jejuni And Campylobacter Coli Genotypes In Poultry Flocks By Restriction Fragment Length Polymorphism (RFLP) Analysis

Ana Claudia Carreira and Monica V. Cunha

23. Pulsed-Field Gel Electrophoresis (PFGE): Application In Population Structure Studies Of Bovine Mastitis-Causing Streptococci

Ilda Santos-Sanches, Lelia Chambel, and Rogerio Tenreiro

24. Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR And Multicolour Capillary Electrophoresis: Application To The Genotyping Of Brucella Species

Giuliano Garofolo

25. Multilocus Sequence Typing (MLST): Markers For The Traceability Of Pathogenic Leptospira Strains

Ahmed Ahmed, Ana S. Ferreira, and Rudy A. Hartskeerl

26. Single Nucleotide Polymorphisms Discrimination Using High-Resolution Melting Analysis For The Genotyping Of Bacillus Anthracis

Sylviane Derzelle

27. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Analysis Of Members Of The Mycobacterium Tuberculosis Complex

Ana Botelho, Ana Canto, Celia Leao, and Monica V. Cunha

28. Rapid Microarray-Based Genotyping Of Chlamydia Spp. Strains From Clinical Tissue Samples

Konrad Sachse and Anke Ruettger

29. Multiplexed Genotyping Of Bacillus Anthracis By Luminex Xmap Suspension Array

Simon Thierry and Sylviane Derzelle

PART IV - Integrative Omics and High-Throughput Platforms to Unravel the Biology of Pathogens and Their Interaction With the Host

30. Next-Generation Sequencing In Veterinary Medicine: How Can The Massive Amount Of Information Arising From High-Throughput Technologies Improve Diagnosis, Control and Management Of Infectious Diseases?

Steven Van Borm, Sandor Belak, Graham Freimanis, Alice Fusaro, Fredrik Granberg, Dirk Hoeper, Donald P. King, Isabella Monne, Richard Orton, and Toon Rosseel

31. Impact Of Next-Generation Technologies On Exploring Socio-Economically Important Parasites And Developing New Interventions

Cinzia Cantacessi, Andreas Hofmann, Bronwyn E. Campbell, and Robin B. Gasser

32. Functional Genomics Of Tick Vectors Challenged With The Cattle Parasite Babesia Bigemina

Ana Domingos, Sandra Antunes, Margarita Villar, and Jose de la Fuente33. Metagenomic Approaches To Disclose Disease-Associated Pathogens: Detection Of Viral Pathogens In Honeybees

Fredrik Granberg, Oskar E. Karlsson, and Sandor Belak

34. Proteomics Characterization Of Tick-Host-Pathogen Interactions

Marina Popara, Margarita Villar, and Jose de la Fuente

Additional information

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies by Monica V. Cunha
Humana Press Inc.
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