1. Applications of Molecular Genetics to the Study of Asthma
Catalina S. Sanz Lozano, Virginia Garcia-Solaesa, Ignacio Davila Gonzalez, and Maria Isidoro-Garcia
2. Assessment and Validation of New Genetic Variants: A systematic In Silico Approach Belen Garcia Berrocal, Asuncion Garcia-Sanchez, and Maria Isidoro-Garcia
3. Introduction to the Gene Expression Analysis Ignacio San Segundo-Val and Catalina S. Sanz Lozano
4. Real Time PCR for Gene Expression Quantification in Asthma
Ignacio San Segundo-Val, Virginia Garcia-Solaesa, and Asuncion Garcia-Sanchez
5. Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma
Ignacio San Segundo-Val and Catalina S. Sanz Lozano
6. Review of Methods to Study Gene Expression Regulation Applied to Asthma
Asuncion Garcia-Sanchez and Fernando Marques Garcia
7. Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma
Virginia Garcia-Solaesa and Catalina S. Sanz Lozano
8. SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma
Virginia Garcia-Solaesa and Sara Ciria Abad
9. Chromatin Immunoprecipitation: Application to the Study of Asthma
Asuncion Garcia-Sanchez and Fernando Marques-Garcia
10. Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols
Asuncion Garcia-Sanchez and Fernando Marques-Garcia
11. Protocols for Exosome Isolation and RNA Profiling
Fernando Marques-Garcia and Maria Isidoro-Garcia
12. Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell
Elena Marcos-Vadillo and Asuncion Garcia-Sanchez
13. Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
Elena Marcos-Vadillo and Asuncion Garcia-Sanchez
14. Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line
Elena Marcos-Vadillo and Asuncion Garcia-Sanchez
15. Review of Mouse Models Applied to the Study of Asthma
Fernando Marques-Garcia and Elena Marcos-Vadillo
16. General and Specific Mouse Models for Asthma Research
Fernando Marques-Garcia and Elena Marcos-Vadillo
17. Mouse Models applied to the Research of Pharmacological Treatments in Asthma
Fernando Marques-Garcia and Elena Marcos-Vadillo
18. Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma
Almudena Sanchez-Martin, Asuncion Garcia-Sanchez, and Maria Isidoro-Garcia