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P-Type ATPases Maike Bublitz

P-Type ATPases By Maike Bublitz

P-Type ATPases by Maike Bublitz


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P-Type ATPases Summary

P-Type ATPases: Methods and Protocols by Maike Bublitz

This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.

Table of Contents

1. An Introduction to P-type ATPase research

Poul Nissen

Part I: Protein Production, Isolation, Purification, and Stabilization

2. Purification of Na,K-ATPase from Pig Kidney

Natalya U. Fedosova

3. Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum

Jesper V. Moller and Claus Olesen

4. Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs

Kazuhiro Abe and Claus Olesen

5. Overproduction of PiB-type ATPases

Xiangyu Liu, Oleg Sitsel, Kaituo Wang, and Pontus Gourdon

6. Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex

Hassina Azouaoui, Cedric Montigny, Aurore Jacquot, Raphaelle Barry, Philippe Champeil, and Guillaume Lenoir

7. The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein

Verena Niggli and Ernesto Carafoli

8. Expression of Na,K-ATPase and H,K-ATPase Isoforms With The Baculovirus Expression System

Jan B. Koenderink and Herman G.P. Swarts

9. Time-dependent Protein Thermostability Assay

Ilse Vandecaetsbeek and Peter Vangheluwe

Part II : Activity Assays

10. Colorimetric Assays of Na,K-ATPase

Kathleen J. Sweadner

11. ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay Pankaj Sehgal, Claus Olesen, and Jesper V. Moller

12. Antimony-Phosphomolybdate ATPase Assay

Gianluca Bartolommei and Francesco Tadini-Buoninsegni

13. ATPase Activity Measurements using radiolabeled ATP

Herman G.P. Swarts and Jan B. Koenderink

14. Assaying P-type ATPases Reconstituted in Liposomes

Hans-Jurgen Apell and Bojana Damnjanovic

15. Coupling ratio for Ca2+ Transport by Calcium Oxalate Precipitation

Pankaj Sehgal, Claus Olesen, and Jesper V. Moller

16. Calcium Uptake in Crude Tissue Preparation

Philip A. Bidwell and Evangelia G. Kranias

17. Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems

Alex Green Wielandt, Michael G. Palmgren, Anja Thoe Fuglsang, Thomas Gunther Pomorski, and Bo Hojen Justesen

18. Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives Magdalena Marek and Thomas Gunther Pomorski

Part III : In vitro Functional Studies

19. The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases

Stefania J. Danko and Hiroshi Suzuki

20. Phosphorylation/Dephosphorylation Assays

Hiroshi Suzuki

21. Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function

Pankaj Sehgal, Claus Olesen, and Jesper V. Moller

Part IV: Ligand Binding Studies

22. Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling

Johannes D. Clausen, David B. McIntosh, David G. Woolley, and Jens Peter Andersen

23. Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+

Pankaj Sehgal, Claus Olesen, and Jesper V. Moller

24. Assay of Copper Transfer and Binding to P1B-ATPases

Teresita Padilla-Benavides and Jose M. Arguello

Part V: Electrophysiology

25. Voltage Clamp Fluorometry of P-Type ATPases

Robert E. Dempski

26. Electrophysiological Measurements on Solid Supported Membranes

Francesco Tadini-Buoninsegni and Gianluca Bartolommei

27. Electrophysiological Characterization of Na,K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping

Florian Hilbers and Hanne Poulsen

Part VI: Functional Studies by Cell Culture and Transgenic Animals

28. Functional Studies of Na+,K+-ATPase using Transfected Cell Cultures

Elena Arystarkhova and Kathleen J. Sweadner

29. HPLC Neurotransmitter Analysis

Thomas Hellesoe Holm, Toke Jost Isaksen, and Karin Lykke-Hartmann

30. Behavior Test Relevant to a2/a3Na+/K+-ATPase Gene Modified Mouse Models

Toke Jost Isaksen, Thomas Hellesoe Holm, and Karin Lykke-Hartmann

31. Zebrafish Whole-mount in situ Hybridization Followed by Sectioning

Canan Doganli, Jens Randel Nyengaard, and Karin Lykke-Hartmann

32. Whole-mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 days Post Fertilization (dpf)) Canan Doganli

Lucas Bukata and Karin Lykke-Hartmann

33. Cell-based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives

Maria S. Jensen, Sara Costa, Thomas Gunther-Pomorski, and Rosa L. Lopez-Marques

34. Transient Expression of P-type ATPases in Tobacco Epidermal Cells

Lisbeth R. Poulsen, Michael G. Palmgren, and Rosa L. Lopez-Marques

Part VII: Lipid Techniques

35. Lipid Exchange by Ultracentrifugation

Nikolaj D. Drachmann and Claus Olesen

36. Reconstitution of Na+, K+-ATPase in Nanodiscs

Jonas Lindholt Gregersen, Natalya Fedosova, Poul Nissen, and Thomas Boesen

Part VIII: Crystallization

37. Crystallization of P-type ATPases by the High Lipid-detergent (HiLiDe) Method

Oleg Sitsel, Kaituo Wang, Xiangyu Liu, and Pontus Gourdon

38. Two-dimensional Crystallization of the Ca2+-ATPase for Electron Crystallography John Paul Glaves, Joseph O. Primeau, and Howard S. Young

39. Two-dimensional Crystallization of Gastric H+,K+-ATPase for Structural Analysis By Electron Crystallography

Kazuhiro Abe

Part IX: Computational Approaches in analyzing P-type ATPases

40. MD Simulations of P-type ATPases in a Lipid Bilayer System

Henriette Elisabeth Autzen and Maria Musgaard

41. Computational Classification of P-Type ATPases

Dan Sondergaard, Michael Knudsen, and Christian Norgaard Storm Pedersen

42. Molecular Modeling of Fluorescent SERCA Biosensors

Bengt Svensson, Joseph M. Autry, and David D. Thomas

43. How to Compare, Analyze and Morph Between Crystal Structures of Different Conformations: The P-type ATPase Example

Jesper L. Karlsen and Maike Bublitz

Additional information

NLS9781493979950
9781493979950
1493979957
P-Type ATPases: Methods and Protocols by Maike Bublitz
New
Paperback
Humana Press Inc.
2019-03-29
544
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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