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PCR Lucilia Domingues

PCR By Lucilia Domingues

PCR by Lucilia Domingues


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PCR Summary

PCR: Methods and Protocols by Lucilia Domingues

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

Table of Contents

1. In silico PCR Tools for a Fast Primer, Probe and Advanced Searching

Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin

2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis

Ruslan Kalendar and Timofey V. Tselykh

3. Long Fragment Polymerase Chain Reaction

Eng Wee Chua, Simran Maggo, and Martin A. Kennedy

4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR

Maria Jorge Campos and Alberto Quesada

5. Inverse PCR for Point Mutation Introduction

Diogo Silva, Gustavo Santos, Mario Barroca, and Tony Collins

6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR

Tatiana Q. Aguiar, Carla Oliveira, and Lucilia Domingues

7. A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli

Ana Filipa Sequeira, Joana L.A. Bras, Vania O. Fernandes, Catarina I.P.D. Guerreiro, Renaud Vincentelli, and Carlos M.G.A. Fontes

<8. Colony PCR

Flavio Azevedo, Humberto Pereira, and Bjoern Johansson

9. CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water

Luc Bissonnette, Andree F. Maheux, and Michel G. Bergeron

10. Multiplex Detection Of Food-borne Pathogen

German Villamizar-Rodriguez and Felipe Lombo

11. Fast Real-time PCR for the Detection of Crustacean Allergens in Foods

Francisco J. Santaclara and Montserrat Espineira

12. Fast Real-time PCR Method for the Detection of Soy in Foods

Montserrat Espineira and Francisco J. Santaclara

13. RAPD/SCAR Approaches for Identification of Adulterant's Breed's Milk in Dairy Products

Joana Cunha and Lucilia Domingues

14. Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers

Farina Mujeeb, Preeti Bajpai, Neelam Pathak, and Smita Rastogu Verma

15. PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism

Laura Vilarinho and Celia Nogueira

16. Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities

S.S. Phadke, A.F. Salvador, J.I. Alves, O. Bretschger, M.M. Alves, and M.A. Pereira

17. PCR in Metagenomics

Sarita G Bhat and Tina K. J.

18. Arbitrarily Primed PCR for Comparison of Metagenomes and Extracting useful loci from them

Leigh Burgoyne, Lin Y Koh, and David Catcheside

Additional information

NLS9781493983810
9781493983810
1493983814
PCR: Methods and Protocols by Lucilia Domingues
New
Paperback
Springer-Verlag New York Inc.
2018-08-08
282
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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