Part I: Introduction
1. What is Synthetic Biology?
Karen M Polizzi
Part II: DNA Assembly
2. Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program
Gang Li, Bing-Xue Dong, Yu-Huan Liu, Chang-Jie Li, and Li-Ping Zhang
3. BioBrick (TM) Assembly Using the In-Fusion PCR Cloning Kit
Sean C. Sleight and Herbert M. Sauro
4. Overlap Extension PCR Cloning
Anton Bryksin and Ichiro Matsumura
5. One-Step Isothermal Assembly of DNA Fragments
Rui TL Rodrigues and Travis S Bayer
PartIII: Part, Pathway, and Genome Engineering
6. Creation and Characterization of Component Libraries for Synthetic Biology
Tim Weenink and Tom Ellis
7. In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements
James Chappell and Paul Freemont
8. In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli
Vandana Sharma and Yohei Yokobayashi
9. Construction and Engineering of Large Biochemical Pathways via DNA Assembler
Zengyi Shao and Huimin Zhao
10. Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors
Peng Xu and Mattheos A.G. Koffas
11. Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research
Matthias Haffke, Cristina Viola, Yan Nie, and Imre Berger
12. Combinatorial DNA Assembly using Golden Gate Cloning
Carola Engler and Sylvestre Marillonnet
13. Construction of Synthetic Gene Circuits in the Escherichia coli Genome
Bei-Wen Ying, Yuya Akeno, and Tetsuya Yomo
14. Shuffling of DNA Cassettes in a Synthetic Integrin
David Bikard and Didier Mazel
Part IV: Computational Tools for Modelling Biological Systems
15. Systematic Methodology for the Development of Mathematical Models for Biological Processes
Cleo Kontoravdi
16. SOBOLHDMR: A General Purpose Modelling Software
Dr Sergei Kucherenko
