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Proteomics in Systems Biology Joerg Reinders

Proteomics in Systems Biology By Joerg Reinders

Proteomics in Systems Biology by Joerg Reinders


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Summary

This volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology.

Proteomics in Systems Biology Summary

Proteomics in Systems Biology: Methods and Protocols by Joerg Reinders

This volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Proteomics in Systems Biology: Methods and Protocols is a valuable resource for researchers who are interested in using proteomics techniques to help answer biological and medical questions.

About Joerg Reinders

Dr. Joerg Reinders Institute of Functional Genomics University Regensburg Joseph-Engert-Strasse 9 93053 Regensburg Germany Phone: +49 941 943 5004 Fax: +49 941 943 5019 E-Mail: [email protected]

Table of Contents

1. Multiplexed Quantitative Proteomics for High-Throughput Comprehensive Proteome Comparisons of Human Cell Lines
Amanda Edwards and Wilhelm Haas
2. Sample Preparation Approaches for iTRAQ Labelling and Quantitative Proteomic Analyses in Systems Biology
Christos Spanos and J. Bernadette Moore
3. Two Birds with One Stone: Parallel Quantification of Proteome and Phospho-Proteome Using iTRAQ
Fiorella A. Solari, Laxmikanth Kollipara, Albert Sickmann, and Rene P. Zahedi
4. Selected Reaction Monitoring to Measure Proteins of Interest in Complex Samples: A Practical Guide
Yuehan Feng and Paola Picotti
5. Monitoring PPARG Induced Changes in Glycolysis by Selected Reaction Monitoring Mass Spectrometry
Andreas Hentschel and Robert Ahrends
6. A Targeted MRM Approach for Tempo-Spatial Proteomics Analyses
Annie Moradian, Tanya R. Porras-Yakushi, Michael J. Sweredoski, and Sonja Hess

7.. Targeted Phospho-Proteome Analysis Using Selected/Multiple Reaction Monitoring (SRM/MRM)
Jun Adachi, Ryohei Narumi, and Takeshi Tomonaga
8. Testing Suitability of Cell Cultures for SILAC-Experiments Using SWATH-Mass Spectrometry
Yvonne Reinders, Daniel Voeller, Anja-K. Bosserhoff, Peter J. Oefner, and Joerg Reinders
9. Combining Amine-Reactive Cross-Linkers and Photo-Reactive Amino Acids for 3D-Structure Analysis of Proteins and Protein Complexes
Philip Loessl and Andrea Sinz
10. Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides
Birte Beine, Hanna C. Diehl, Helmut E. Meyer, and Corinna Henkel
11. Ethyl Esterification for MALDI-MS Analysis of Protein Glycosylation
Karli R. Reiding, Emanuela Lonardi, Agnes L. Hipgrave Ederveen, and Manfred Wuhrer
12. Characterization of Protein N-Glycosylation by Analysis of ZIC-HILIC-Enriched Intact Proteolytic Glycopeptides
Gottfried Pohlentz, Kristina Marx, and Michael Mormann
13. Simple and Effective Affinity Purification Procedures for Mass Spectrometry-Based Identification of Protein-Protein Interactions in Cell Signaling Pathways
Julian H. M. Kwan and Andrea Emili
14. A Systems Approach to Understand Antigen Presentation and the Immune Response
Nadine L. Dudek, Nathan P. Croft, Ralf B. Schittenhelm, Sri H. Ramarathinam, and Anthony W. Purcell15. Profiling of Small Molecules by Chemical Proteomics
Kilian HUber and Giulio Superti-Furga
16. Generating Sample Specific Databases for Mass Spectrometry Based Proteomic Analysis by Using RNA Sequencing
Toni Luge and Sascha Sauer
17. A Proteomic Workflow Using High Throughout De Novo Sequencing towards Complementation of Genome Information for Improved Comparative Crop Science
Reinhard Turetschek, David Lyon, Getinet Desalegn, Hans-Peter Kaul, and Stefanie Wienkoop
18. From Phospho-Proteome to Modelling of Plant Signalling Pathways
Maksim Zakhartsev, Heidi Pertl-Obermeyer, and Waltraud X. Schulze
19. Interpretation of Quantitative Shotgun Proteomic DataElise Aasebo, Frode S. Berven, Frode Selheim, Harald Barsnes, and Marc Vaudel
20. A Simple Workflow for Large Scale Shotgun Glycoproteomics
Astrid Guldbrandsen, Harald Barsnes, Ann Cathrine Kroksveen, Frode A. Berven, and Marc Vaudel
21. Systemic Analysis of Regulated Functional Networks
Luis Francisco Hernandez Sanchez, Elise Aasebo, Frode Selheim, Frode S. Berven, Helge Raeder, Harald Barsnes, and Marc Vaudel

Additional information

NLS9781493980253
9781493980253
1493980254
Proteomics in Systems Biology: Methods and Protocols by Joerg Reinders
New
Paperback
Humana Press Inc.
2019-03-27
313
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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