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High-Throughput Phenotyping in Plants Jennifer Normanly

High-Throughput Phenotyping in Plants By Jennifer Normanly

High-Throughput Phenotyping in Plants by Jennifer Normanly


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Summary

This Methods in Molecular Biology(TM) book is written on the premise that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. Offers materials lists, protocols and troubleshooting tips.

High-Throughput Phenotyping in Plants Summary

High-Throughput Phenotyping in Plants: Methods and Protocols by Jennifer Normanly

Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, High Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.

Table of Contents

1. Image-Based Analysis of Light-Grown Seedling Hypocotyls in Arabidopsis

Benjamin J. Cole and Joanne Chory

2. High Throughput Phenotyping of Plant Shoots

Bettina Berger, Bas de Regt, and Mark Tester

3. High Throughput Phenotyping of Root Growth Dynamics

Nima Yazdanbakhsh and Joachim Fisahn

4. LEAF GUI: Analyzing the Geometry of Veins and Areoles Using Image Segmentation Algorithms

Charles A. Price

5. Remote Chlorophyll Fluorescence Measurements with the Laser Induced Fluorescence Transient (LIFT) Approach

Roland Pieruschka, Denis Klimov, Joseph A. Berry, C. Barry Osmond, Uwe Rascher, and Zbigniew S. Kolber

6. Leaf Hue Measurements: A High Throughput Screening of Chlorophyll Content

Laszlo Sass, Petra Majer, and Eva Hideg

7. High Resolution, Time-Lapse Imaging for Ecosystem-Scale Phenotyping in the Field

Tim Brown, Christopher Zimmermann, Whitney Panneton, Nina Noah, and Justin Borevitz

8. High Throughput Phenotyping of Plant Populations Using a Personal Digital Assistant

Raju Naik Vankudavath, Reddaiah Bodanapu, Yellamaraju Sreelakshmi, and Rameshwar Sharma

9. High Throughput Fractionation of Natural Products for Drug Discovery

Ying Tu and Bing Yan

10. Conducting Molecular Biomarker Discovery Studies in Plants

Christian Schudoma, Matthias Steinfath, Heike Sprenger, Joost T. van Dongen, Dirk Hincha, Ellen Zuther, Peter Geigenberger, Joachim Kopka, Karin Koehl, and Dirk Walther

11. Highly Sensitive High Throughput Profiling of Six Phytohormones Using MS-Probe Modification and Liquid Chromatography-Tandem Mass Spectrometry

Mikiko Kojima and Hitoshi Sakakibara

12. Qualitative and Quantitative Screening of Amino Acids in Plant Tissues

Will I. Menzel, Wen-Ping Chen, Adrian D. Hegeman, and Jerry D. Cohen

13. Arabidopsis thaliana Membrane Lipid Molecular Species and Their Mass Spectral Analysis

Thilani Samarakoon, Sunitha Shiva, Kaleb Lowe, Pamela Tamura, Mary R. Roth, and Ruth Welti

14. ICP-MS as a Tool For High Throughput Analysis of Plants

Javier Seravalli

15. The Plant Volatilome: Methods of Analysis

Carlo Bicchi and Massimo Maffei

16. High Throughput Monitoring of Plant Nuclear DNA Contents via Flow Cytometry

David W. Galbraith and Georgina M. Lambert

17. Transient RNAi Assay in 96-Well Plate Format Facilitates High Throughput Gene Function Studies in planta

Shu-Zon Wu and Magdalena Bezanilla

18. A High Throughput Biological Conversion Assay for Determining Lignocellulosic Quality

Scott J. Lee, Thomas A. Warnick, Susan B. Leschine, and Samuel P. Hazen

19. Carbohydrate Microarrays in Plant Science

Jonatan U. Fangel, Henriette L. Pedersen, Silvia Vidal Melgosa, Louise I. Ahl, Armando Asuncion Salmean, Jack Egelund, Maja Gro Rydahl, Mads H. Clausen, and William G.T. Willats

Additional information

NLS9781493959457
9781493959457
149395945X
High-Throughput Phenotyping in Plants: Methods and Protocols by Jennifer Normanly
New
Paperback
Humana Press Inc.
2017-04-30
365
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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