Part I: Membrane Protein Purification 1. Characterization of Membrane Protein Preparations: Measurement of Detergent Content and Ligand Binding after Proteoliposomes Reconstitution Mariano A. Ostuni, Soria Iatmanen, David Teboul, Jean-Claude Robert, and Jean-Jacques Lacapere 2. Native Membrane Proteins Versus Yeast Recombinant: An Example, the Mitochondrial ADP/ATP Carrier Bertrand Arnou, Cecile Dahout-Gonzalez, Ludovic Pelosi, Guy J.-M. Lauquin, Gerard Brandolin, and Veronique Trezeguet 3. Bacterial Overexpressed Membrane Proteins: An Example, the TSPO Jean-Claude Robert and Jean-Jacques Lacapere 4. Insect Cell Versus Bacterial Overexpressed Membrane Proteins: An Example, the Human ABCG2 Transporter Alexandre Pozza, Jose M. Perez-Victoria, and Attilio Di Pietro Part II: X-Ray Crystallography 5. Crystallography of Membrane Proteins: From Crystallization to Structure Aurelien Deniaud, Ekaterina Moiseeva, Valentin Gordeliy, and Eva Pebay-Peyroula 6. Structural Approaches of the Mitochondrial Carrier Family Hughes Nury, Iulia Blesneac, Stephanie Ravaud, and Eva Pebay-Peyroula 7. What Can Be Learned about the Function of a Single Protein from Its Various X-Ray Structures: The Example of the Sarcoplasmic Calcium Pump Jesper Vuust Moller, Claus Olesen, Anne-Marie Lund Winther, and Poul Nissen 8. Toward Drug Design: Recent Progress in the Structure of GPCR, a Membrane Protein with High Potential as a Pharmaceutical Target Vadim Cherezov, Enrique Abola, and Raymond C. Stevens Part III: Electron Microscopy 9. Observation of Membrane Proteins in situ: AQPcic, the Insect Aquaporin Example Daniel Thomas and Annie Cavalier 10. Two-Dimensional Crystallization of Integral Membrane Proteins for Electron Crystallography David L. Stokes, William J. Rice, Minghui Hu, Changki Kim, and Iban Ubarretxena 11. Structure Determination of Membrane Protein by Both Cryo Electron Tomography and Single Particle Analysis Sylvain Trepout, Jean-Christophe Taveau,and Olivier Lambert 12. Electron Microscope Tomography of Native Membranes Gabriel Peranzi, Cedric Messaoudi, Leeyah Issop, and Jean-Jacques Lacapere 13. From Electron Microscopy Maps to Atomic Structures Using Normal Mode-Based Fitting Konrad Hinsen, Edward Beaumont, Bertrand Fournier, and Jean-Jacques Lacapere Part IV: Nuclear Magnetic Resonance 14. Determination of Membrane Protein Structures Using Solution and Solid-State NMR Pierre Montaville and Nadege Jamin 15. Membrane Protein Fragments Reveal Both Secondary and Tertiary Structure of Membrane Proteins Philip L. Yeagle and Arlene D. Albert 16. What Can We Learn From a Small Regulatory Membrane Protein? Gianluigi Veglia, Kim N. Ha, Lei Shi, Raffaello Verardi, and Nathaniel J. Traaseth 17. Solution-State NMR Spectroscopy for Investigating 3D Structure of Membrane Proteins in Detergent Micelles: Structure of the Klebsiella pneumoniae Outer Membrane Protein, KpOmpA Marie Renault, Olivier Saurel, Pascal Demange, Virginie Reat, and Alain Milon 18. NMR Spectroscopy of Lipid Bilayers Axelle Grelard, Cecile Loudet, Anna Diller, and Erick J. Dufourc Part V: Molecular Modelling 19. Critical Review of General Guidelines for Membrane Proteins Model Building and Analysis Catherine Etchebest and Gaelle Debret 20. 3D-Structural Models of Transmembrane Proteins Alexandre G. de Brevern 21. Molecular Dynamics of Membrane Peptides and Proteins: Principles and Comparison to Experimental Data Patrick F.J. Fuchs 22. Membrane Protein Dynamics from Femtoseconds to Seconds Christian Kandt and Luca Monticelli 23. The Family of G Protein-Coupled Receptors: An Example of Membrane Proteins Irina G. Tikhonova and Daniel Fourmy
