Section 1: Microfluidic Diagnostics: From the Classroom to the Boardroom
1. Present Technology and Future Trends in Point of Care Microfluidic Diagnostics
Lawrence Kulinsky, Zahra Noroozi, and Marc Madou
2. Teaching Microfluidic Diagnostics Using Jell-O Chips
Cheng Wei T. Yang and Eric T. Lagally
3. Fundamentals of Microfluidics for High School Students with No Prior Knowledge of Fluid Mechanics
Vishal Tandon and Walter Peck
4. Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method
Chenren Shao and Don L. DeVoe
5. Overview of the Microfluidic Diagnostics Commercial Landscape
L. Kim
6. Practical Aspects of the Preparation and Filing of Patent Applications
Fiona Bessoth
7. Introduction to In Vitro Diagnostic Device Regulatory Requirements
Jonathan Day
Section 2. Microfluidic Diagnostics: Fabrication and Manipulation Protocols
8. Microfluidic Device Fabrication by Thermoplastic Hot-Embossing
Shuang Yang and Don L. DeVoe
9. Introduction to Glass Microstructuring Techniques
Radoslaw Mazurczyk, Colin Mansfield, Marcin Lygan
10.Glass Microstructure Capping and Bonding Techniques
Radoslaw Mazurczyk, Colin Mansfield, and Marcin Lygan
11. Rapid Prototyping of PDMS Device using SU-8 Lithography
Gareth Jenkins
12. Microfluidic Interface Technology Based on Stereolithography for Glass-based Lab-on-a-Chips
Song-I Han and Ki-Ho Han
13. Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays
Scott T. Phillips and Nicole K. Thom
14. Thread Based Devices for Low-Cost Diagnostics
Meital Reches
15. Droplet Based Microfluidics
Sanjiv Sharma, Monphichar Srisa-Art, Steven Scott, Amit Asthana, and Anthony Cass
16. Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET
Monipchar Srisa-Art and Sanjiv Sharma
17. Surface Treatments for Microfluidic Biocompatibility
N.J. Shirtcliffe and P. Roach
18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces
N.J. Shirtcliffe, and P. Roach
Section 3. Microfluidic Diagnostics: Application Protocols
19. The Application of Microfluidic Devices for Viral Diagnosis in Developing Countries
Samantha M. Hattersley, John Greenman, and Stephen J. Haswell
20. Applications of Microfluidics for Molecular Diagnostics
Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale
21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethysiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers
Peng Li
22. Breast Cancer Diagnostics using Microfluidic Multiplexed Immunohistochemistry
Minseok S. Kim, Seyong Kwon, and Je-Kyun Park
23. Charged-couple Device (CCD) Detectors for Lab-on-a Chip (LOC) Optical Analysis
Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck
24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels
Michael P. Cuchiara and Jennifer L. West
25. Purifications of DNA/RNA in a Microfluidic Device
Andy Fan, Samantha Byrnes, and Catherine Klapperich
26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR
Xuefei Leng and Chaoyong James Yang
27. Intergrated Fluidic Circuits (IFCs) for Digital PCR
Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver
28. microFIND (R) Approach to Fluorescent in Situ Hybridization (FISH)
Andrea Zanardi, Emanuele Barborini, and Roberta Carbone
29. An ELISA Lab-on-a-CHOP (ElISA-LOC)
Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov
30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis
Eric Ouellet, Louise Lund, and Eric T. Lagally
31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement
Kerstin Lange, Friederike J. Gruhl, and Michael Rapp
32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins
Junjie Ou, Carolyn L. Ren
