Part I: Protein Assembly and Trafficking
1. Assaying AMPA Receptor Oligomerization
Catherine L. Salussolia, Quan Gan, and Lonnie P. Wollmuth
2. A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells
Mark R. P. Aurousseau, Hugo McGuire, Rikard Blunck, and Derek Bowie
3. Counting NMDA Receptors at the Cell Surface
Martin Horak and Young Ho Suh
4. Electron Microscopy Analysis of AMPA receptors in Dendritic Spines
Audra A. Kramer, Amber N. Petersen, and Nashaat Z. Gerges
5. Electrophysiological Tagging of Ionotropic Receptors
Andres Barria
6. Functional Detection of Novel Triheteromeric NMDA Receptors
Sanjay S. Kumar
Part II: Protein Structure and Dynamics
7. Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses
Kelly A. Krogh and Stanley A. Thayer
8. NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains
Christopher P. Ptak, Ahmed H. Ahmed, and Robert E. Oswald
9. Computing Conformational Free Energies of iGluR Ligand-Binding Domains
Alvin Yu, Tyler Wied, John Belcher, and Albert Y. Lau
10. LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors
Rita E. Sirrieh and Vasanthi Jayaraman
11. Assaying the Energetics of NMDA Receptor Pore Opening
Rashek Kazi, Melissa Daniel, and Lonnie P. Wollmuth
Part III: Protein Function
12. Constructing a Rapid Solution Exchange System
David M. MacLean
13. Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics
Mark W. Fleck
14. Analysis of Whole-Cell NMDA Receptor Responses
Vojtech Vyklicky, Miloslav Korinek, Ales Balik, Tereza Smejkalova, Barbora Krausova, and Ladislav Vyklicky
15. Expression, Purification, and Crystallization of Full Length
Ionotropic Glutamate Receptors
Maria V. Yelshanskaya, Kei Saotome, Minfen Li, and Alexander I. Sobolevsky
16. Timing AMPA Receptor Activation with Laser-pulse Photolysis
Li Niu
17. Current Recording and Kinetic Analyses for Single AMPA Receptors
Kinning Poon, Robert E. Oswald, and Linda M. Nowak
18. Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings
Kirstie A. Cummings, Gary J. Iacobucci, Gabriela K. Popescu
