This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols.

Authoritative and cutting-edge,Plant Secondary Metabolism Engineering: Methods and Protocols, Second Editionaimsto be auseful practical guide to help researchers working in this exciting field.