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Extracellular Vesicles By Winston Patrick Kuo

Extracellular Vesicles by Winston Patrick Kuo

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Summary

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling.

Extracellular Vesicles Summary

Extracellular Vesicles: Methods and Protocols by Winston Patrick Kuo

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Extracellular Vesicles: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications.

Table of Contents

1. Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine

Mingyu Shang, John Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo, and Hongjun Kang

2. Red Blood Cells: A Source of Extracellular Vesicles

Winston Patrick Kuo, John Tigges, Vasilis Toxavidis, and Ionita Ghiran

Part I: Isolation of Extracellular Vesicles

3. Isolation of Extracelullar Vesicles by Ultracentrifugation

Fatemeh Momen-Heravi

4. Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles

Mitja L. Heinemann and Jody Vykoukal

5. Paper-Based Devices for Isolation of Extracellular Vesicles

Yi-Hsing Hsiao and Chihchen Chen

6. Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis

Cindy M. Yamamoto, Taku Murakami, and Shu-Wing Ng

7. Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads

Ketil W. Pedersen, Bente Kierulf, and Axl Neurauter

Part II: Purification of Extracellular Vesicles

8. Polymer-Based Purification of Extracellular Vesicles

Peter N. Brown and Hang Yin

9. Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification

Richard Lobb and Andreas Moeller

10. Purification Protocols for Extracellular Vesicles

Rebecca E. Lane, Darren Korbie, Matt Trau, and Michelle M. Hill

Part III: Characterization of Extracellular Vesicles

11. Characterization of Extracellular Vesicles by Surface Plasmon Resonance

Hyungsoon Im, Katherine Yang, Hakho Lee, and Cesar M. Castro

12. Extracellular Vesicle Isolation and Analysis by Western Blotting

Emma J.K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, and George M. Church

13. Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis

Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, and Rebecca Dragovic

14. Characterization of Extracellular Vesicles by Flow Cytometry

Virginia Camacho, Vasilis Toxavidis, and John C. Tigges

15. Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)

Tao Huang and Jiang He

16. Multi-Surface Antigen Staining of Larger Extracellular Vesicles

Veronika Lukacs-Kornek, Henrike Julich, Sabine Katharina Urban, and Miroslaw Kornek

17. Microcapillary Chip-Based Extracellular Vesicle Profiling System

Takanori Akagi and Takanori Ichiki

Part IV: Imaging of Extracellular Vesicles

18. Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy

Petr Cizmar and Yuana Yuana

19. Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy

Dmitry Ter-Ovanesyan, Emma J.K. Kowal, Aviv Regev, George M. Church, and Emanuele Cocucci

Part V: Tracking of Extracellular Vesicles

20. In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase

Yuki Takahashi, Makiya Nishikawa, and Yoshinobu Takakura

21. Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters

Anthony Yan-Tang Wu and Charles Pin-Kuang Lai

Part VI: Downstream Extracellular Vesicle Applications Genomics and Proteomics

22. Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples

Davide Zocco and Natasa Zarovni

23. Extracellular Vesicle miRNA Detection Using Molecular Beacons

Won Jong Rhee and Seunga Jeong

24. Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling

Simion Kreimer and Alexander R. Ivanov

25. An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles

Stephanie N. Hurwitz and David G. Meckes, Jr.

Part VII: Isolation of Extracellular Vesicles from Biofluids

26. Isolation of Extracellular Vesicles from Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits

Shlomit Kenigsberg, Clifford C. Librach, and Juliano C. da Silveira

27. Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation

Kazuya Iwai, Satoshi Yamamoto, Mitsutaka Yoshida, and Kiyotaka Shiba

28. Isolation of Extracellular Vesicles from Breast Milk

Xin Wang

29. An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis

Li-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci, and ShuQi Wang

Part VIII: Extracellular Vesicles Isolated from Cell Culture, Parasites, and Stem Cells

30. Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles

Chris Wang, Austin Wang, Fang Wei, David T.W. Wong, and Michael Tu

31. Production and Characterization of Extracellular Vesicles in Malaria

Smart Mbagwu, Michael Walch, Luis Filgueira, and Pierre-Yves Mantel

32. Isolation of Extracellular Vesicles from Stem Cells

Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal L. Weintraub, and Yaoliang Tang

Part IX: Extracellular Vesicles in Mouse Models

33. The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model

Noemi Garcia-Romero, Gorjana Rackov, Cristobal Belda-Iniesta, and Angel Ayuso-Sacido

Part X: Therapeutic Applications of Extracellular Vesicles

34. Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine

Gareth R. Willis, Stella Kourembanas, and S. Alex Mitsialis

35. Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles

Jing Liu, Jingwei Ma, Ke Tang, and Bo Huang

Additional information

NPB9781493972517
9781493972517
1493972510
Extracellular Vesicles: Methods and Protocols by Winston Patrick Kuo
New
Hardback
Humana Press Inc.
2017-08-21
446
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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