1. Cell Senescence Culturing Methods
Huaping Chen, Yuanyuan Li, and Trygve O. Tollefsbol
2. Digital Image Analysis of Cells Stained with the Senescence-Associated Beta-Galactosidase Assay
Liran I. Shlush and Sara Selig
3. Analysis of Biomarkers of Caloric Restriction in Aging Cells
Yuanyuan Li and Trygve O. Tollefsbol
4. Cell Sorting of Young and Senescent Cells
Graeme Hewitt, Thomas von Zglinicki, and Joao F. Passos
5. Studying the Replicative Life Span of Yeast Cells
David A. Sinclair
6. Methods for Creating Mutations in C. elegans that Extend Lifespan
Dayong Wang, Min Cao, Jessica Dinh, and Yuqing Dong
7. Aging Studies in Drosophila Melanogaster
Yaning Sun, Jason Yolitz, Cecilia Wang, Edward Spangler, Ming Zhan, and Sige Zou
8. The Use of Calorie Restriction Mimetics to Study Aging
Munehiro Kitada and Daisuke Koya
9. Using Somatic-Cell Nuclear Transfer to Study Aging
Satoshi Kishigami, Ah Reum Lee, and Teruhiko Wakayama
10. Induction of Cellular Senescence by Oncogenic RAS
Romi Gupta and Narendra Wajapeyee
11. Methods of Cellular Senescence Induction Using Oxidative Stress
Zhe Wang, Dandan Wie, and Hengyi Xiao
12. Methods of Testing Pharmacological Drugs Effects on Aging and Life-span in Mice
Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski
13. Mitochondria-targeted Anti-aging Gene Therapy with Adeno-associated Viral Vectors
Dejia Li and Dongsheng Duan
14. Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration
Nadav Kimelman Bleich, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli , Zulma Gazit , Gadi Pelled, and Dan Gazit
15. Exometabolomic Mapping of Caenorhabditis elegans - A Tool to Non-Invasively Investigate Aging
Robert J. Mishur, Jeffrey A. Butler, and Shane L. Rea
16. Detecting Polymorphisms in Human Longevity Studies: HLA typing and SNP Genotyping by Amplicon Sequencing
Gilberto Vargas-Alarcon and Carmina Flores-Dominguez
17. Gel Electrophoresis-based Proteomics of Senescent Tissues
Steven Carberry and Kay Ohlendieck
18. Single-Cell Semiconductor Sequencing
Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, and Leonid L. Moroz
19. Application of DNA Microarray Technology to Gerontological Studies
Kiyoshi Masuda, Yuki Kuwano, Kensei Nishida, and Kazuhito Rokutan
20. Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells
Carmen M. Koch and Wolfgang Wagner
21. Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging
Leonid L. Moroz and Andrea B. Kohn
