Quantitative Proteomics by Mass Spectrometry Editor Salvatore Sechi Table of Contents 1. Preface by Salvatore Sechi: An Overview of Quantitative Proteomics by Mass Spectrometry. 2. Illarion V. Turko and Salvatore Sechi: Acrylamide - A Cysteine Alkylating Reagent for Quantitative Proteomics. 3. Jeffrey A. Ranish, Marjorie Brand and Ruedi Aebersold: Using stable isotope tagging and MS to characterize protein complexes and to detect changes in their composition. 4. Shao-En Ong and Matthias Mann: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics. 5. Toshitaka Sato, Yasushi Ishihama, Yoshiya Oda: Quantitative Proteomics of Mouse Brain, and Specific Protein-Interaction Studies using Stable Isotope Labeling. 6. Scott A. Gerber, Arminja N. Kettenbach, John Rush and Steven P. Gygi: The Absolute Quantification Strategy: Application to Phosphorylation Profiling of Human Separase Serine 1126. 7. Sushmita Mimi Roy and Christopher H. Becker: Quantification of proteins and metabolites by mass spectrometry without isotopic labeling. 8. Tao Liu, Wei-Jun Qian, David G. Camp, II, and Richard D. Smith: The Use of a Quantitative Cysteinyl-peptide Enrichment Technology for High-Throughput Quantitative Proteomics. 9. Fred E. Regnier: An isotope coding strategy for proteomics involving both amine and carboxyl group labeling 10. Catherine Fenselau and Xudong Yao: Proteolytic Labeling with 18O for Comparative Proteomics Studies: preparation of 18O-labeled peptides and the 18O/16O peptide mixture 11. Franti_ek Ture_ek, C. Ronald Scott, and Michael H. Gelb: Tandem mass spectrometry in the detection of inborn errors of metabolism and newborn screening. 12. Yu Lu, Patricia Bottari, Ruedi Aebersold, Franti_ek Ture_ek, and Michael H. Gelb: Absolute quantitation of proteins in complex mixtures by the Visible Isotope-Coded Affinity Tagsmethod. 13. Michael J. MacCoss and Christine C. Wu.: Computational Analysis of Quantitative Proteomics Data using Stable Isotope Labeling. 14. Christine C. Wu and Michael J. MacCoss: Quantitative Proteomic Analysis of Mammalian Organisms Using Metabolically Labeled Tissues. 15. Zhang, Y., Wolf-Yadlin A., White F.M.: Quantitative Proteomic Analysis of Phosphotyrosine-Mediated Cellular Signaling Networks.