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Phytoplasma Matt Dickinson

Phytoplasma By Matt Dickinson

Phytoplasma by Matt Dickinson


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Summary

Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply.

Phytoplasma Summary

Phytoplasma: Methods and Protocols by Matt Dickinson

Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.

Table of Contents

1. The Phytoplasmas - An Introduction

Matt Dickinson, Melanie Tuffen, and Jennifer Hodgetts

2. Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus

Jennifer Hodgetts, David Crossley, and Matt Dickinson

3. Micropropagation and Maintenance of Phytoplasmas in Tissue Culture

Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, and Nicoletta Contaldo

4. Dodder Transmission of Phytoplasmas

Jaroslava Pribylova and Josef Spak

5. Insect Maintenance and Transmission

Heather Kingdom

6. Capturing Insect Vectors of Phytoplasmas

Phyllis Weintraub and Jurgen Gross

7. Insect Vector Transmission Assays

Domenico Bosco and Rosemarie Tedeschi

8. Molecular Identification of Phytoplasma Vector Species

Sabrina Bertin and Domenico Bosco

9. Dienes' Staining and Light Microscopy for Phytoplasma Visualization

Rita Musetti

10. DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas

Nancy S. Andrade and Nolberto S. Arismendi

11. Visualisation of Phytoplasmas using Electron Microscopy

B. Jean Devonshire

12. Automated DNA Extraction for Large Numbers of Plant Samples

Natasa Mehle, Petra Nikolic, Matevz Rupar, Jana Boben, Maja Ravnikar, and Marina Dermastia

13. DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts

Nigel A. Harrison, Robert E. Davis, and Ericka E. Helmick

14. Nested PCR and RFLP Analysis Based on the 16S rRNA Gene

Bojan Duduk, Samantha Paltrinieri, Ing Ming Lee, and Assunta Bertaccini

15. PCR and RFLP Analyses Based on the Ribosomal Protein Operon

Marta Martini and Ing-Ming Lee

16. Tuf and secY PCR Amplification and Genotyping of Phytoplasmas

Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Safarova, Pavla Valova, and Milan Navratil

17. PCR Analysis of Phytoplasmas Based on the secA Gene

Matt Dickinson and Jennifer Hodgetts

18. Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains

Martina Seruga Music and Dijana Skoric

19. Microarrays for Universal Detection and Identification of Phytoplasmas

Mogens Nicolaisen, Henriette Nyskjold, and Assunta Bertaccine

20. T-RFLP for Detection and Identification of Phytoplasmas in Plants

Jennifer Hodgetts and Matt Dickinson

21. Real-Time PCR for Universal Phytoplasma Detection and Quantification

Nynne Meyn Christensen, Henriette Nyskjold, and Mogens Nicolaisen

22. A Real-Time PCR Detection System for the Bois Noir and Flavescence Doree Phytoplasmas and Quantification of the Target DNA

Natasa Mehle, Nina Prezelj, Matjaz Hren, Jana Boben, Kristina Gruden, Maja Ravnikar, and Marina Dermastia

23. Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group

Natasa Mehle, Petra Nikolic, Kristina Gruden, Maja Ravnikar, and Marina Dermastia

24. Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions

Paola Margaria and Sabrina Palmano

25. In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP)

Jenny Tomlinson

26. DNA Bar-Coding for Phytoplasma Identification

Olga Makarova, Nicoletta Contaldo, Samanta Paltrinieri, Assunta Bertaccini, Henriette Nyskjold, and Mogens Nicolaisen

27. Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison

Hiromi Nishida

28. The iPhyClassifier, An Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment

Yan Zhao, Wei Wei, Ing-Ming Lee, Jonathan Shao, Xiaobing Suo, and Robert E. Davis

29. Phytoplasma Proteomic Analysis

Xianling Ji and Yingping Gai

30. Preparation of Phytoplasma Membrane Recombinant Proteins

Luciana Galetto, Majid Siampour, and Cristina Marzachi

31. Phytoplasma Plasmid DNA Extraction

Mark T. Andersen and Lia W. Liefting

32. Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA

Lucy T.T. Tran-Nguyen and Bernd Schneider

33. Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants

Carmine Marcone

34. Mapping the Phytoplasma Chromosome

Sylvie Malembic-Maher and Patricia Carle

Additional information

NLS9781493959655
9781493959655
1493959654
Phytoplasma: Methods and Protocols by Matt Dickinson
New
Paperback
Humana Press Inc.
2016-08-23
421
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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