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Stem Cells and Good Manufacturing Practices By Kursad Turksen

Stem Cells and Good Manufacturing Practices by Kursad Turksen

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Stem Cells and Good Manufacturing Practices Summary

Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations by Kursad Turksen

This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Inclusive and authoritative, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations will be an invaluable resource to both basic and clinical practitioners in stem cell biology.

Table of Contents

1. Therapeutic Mesenchymal Stromal Cells: Where We Are Headed

Patrick J. Hanley

2. The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks

Vincent C. Chen and Larry A. Couture

3. Scalable Expansion of Human Induced Pluripotent Stem Cells in Xeno-Free Microcarriers

Sara M. Badenes, Tiago G. Fernandes, Carlos A.V. Rodrigues, Maria Margarida

Diogo, and Joaquim M.S. Cabral

4. Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs

Jens Durruthy-Durruthy and Vittorio Sebastiano

5. Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging

Sebastien Sart, Fabian Calixto Bejarano, Yuanwei Yan, Samuel C. Grant, and Yan Li

6. Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells

Gema Martin-Manso and Patrick J. Hanley

7. Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions

Heba Badraiq, Liani Devito, and Dusko Ilic

8. Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use

Phuc Van Pham and Phan Kim Ngoc

9. Xeno-Free Culture of Human Periodontal Ligament Stem Cells

Oriana Trubiani and Francesca Diomede

10. GMP-Compliant Human Adipose Tissue-Derived Stromal Cells for Cellular Therapy

Hamid-Reza Aghayan, Parisa Goodarzi, and Babak Arjmand

11. cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells

Elena Veronesi, Jorge Sans Burns, Alba Murgia, Olivia Candini, Valeria Rasini,

Ilenia Mastrolia, Fabio Catani, Paolo Paolucci, and Massimo Dominici

12. GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation

Bagher Larijani, Hamid-Reza Aghayan, Parisa Goodarzi, and Babak Arjmand

13. Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting

Goncalo M.C. Rodrigues, Tiago G. Fernandes, Carlos A.V. Rodrigues, Joaquim M.S. Cabral, and Maria Margarida Diogo

14. Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems

Joana G. Carmelo, Ana Fernandes-Platzgummer, Joaquim M.S. Cabral, and Claudia Lobato da Silva

15. Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process

Marta Serra, Livia Roseti, and Alessandra Bassi

16. Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells

Livia Roseti, Marta Serra, and Alessandra Bassi

17. Culture of Human Limbal Epithelial Stem Cells on Tenon's Fibroblast Feeder-Layers: A Translational Approach

Gaia Scafetta, Camilla Siciliano, Giacomo Frati, and Elena De Falco

18. Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements

Christiane L. Elseberg, Denise Salzig, and Peter Czermak

Additional information

NPB9781493924349
9781493924349
1493924346
Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations by Kursad Turksen
New
Hardback
Humana Press Inc.
2015-02-14
220
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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