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Bioreactors in Stem Cell Biology By Kursad Turksen

Bioreactors in Stem Cell Biology by Kursad Turksen

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Summary

This meticulous volume recognizes the need to translate what has been learned primarily in tissue culture dishes to approaches supporting scale-up studies, not only to large quantities of cells but also to heterogeneous cell constructs.

Bioreactors in Stem Cell Biology Summary

Bioreactors in Stem Cell Biology: Methods and Protocols by Kursad Turksen

This meticulous volume recognizes the need to translate what has been learned primarily in tissue culture dishes to approaches supporting scale-up studies, not only to large quantities of cells but also to heterogeneous cell constructs. Notable advances are being made in these latter approaches, prompting this collection of a variety of representative protocols that facilitate important modifications and novel approaches to bioreactors in stem cell research, contributed by both established and new investigators in this area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Bioreactors in Stem Cell Biology: Methods and Protocols will serve as an ideal guide for scientists seeking to increase our understanding of stem cells and their potential to repair and regenerate tissues and organs.

Table of Contents

1. Multi-Compartmental Hollow-Fiber-Based Bioreactors for Dynamic Three-Dimensional Perfusion Culture

Eva Schmelzer and Joerg C. Gerlach

2. A Bioreactor to Apply Multi-Modal Physical Stimuli to Cultured Cells

Jan-Christoph Edelmann, Lizzie Jones, Remi Peyronnet, Liang Lu, Peter Kohl, and Ursula Ravens

3. Aggregate and Microcarrier Cultures of Human Pluripotent Stem Cells in Stirred Suspension Systems

Preeti Ashok, Yongjia Fan, Mahboubeh R. Rostami, and Emmanuel S. Tzanakakis

4. Expansion of Human Induced Pluripotent Stem Cells in Stirred Suspension Bioreactors

Walaa Almutawaa, Leili Rohani, and Derrick E. Rancourt

5. Uniformed Embryoid Body Production and Enhanced Mesendoderm Differentiation with Murine Embryonic Stem Cells in a Rotary Suspension Bioreactor

Xiaohua Lei, Zhili Deng, and Enkui Duan

6. Expansion of Human Mesenchymal Stem Cells in a Microcarrier Bioreactor

Ang-Chen Tsai and Teng Ma

7. Large-Scale Expansion and Differentiation of Mesenchymal Stem Cells in Microcarrier-Based Stirred Bioreactors

Sebastien Sart and Spiros N. Agathos

8. Bioreactor Expansion of Skin-Derived Precursor Schwann Cells

Tylor Walsh, Jeff Biernaskie, Rajiv Midha, and Michael S. Kallos

9. Use of Stirred Suspension Bioreactors for Male Germ Cell Enrichment

Sadman Sakib, Camila Dores, Derrick Rancourt, and Ina Dobrinski

10. Generation of Neural Progenitor Spheres from Human Pluripotent Stem Cells in a Suspension Bioreactor

Yuanwei Yan, Liqing Song, Ang-Chen Tsai, Teng Ma, and Yan Li

11. Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells

Daniel Simao, Francisca Arez, Ana P. Terasso, Catarina Pinto, Marcos F.Q. Sousa, Catarina Brito, and Paula M. Alves

12. Scalable Expansion of Human Pluripotent Stem Cell-Derived Neural Progenitors in Stirred Suspension Bioreactor Under Xeno-Free Condition Shiva Nemati, Saeed Abbasalizadeh, and Hossein Baharvand

13. A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies

Jonas Christoffersson, Gunnar Bergstroem, Kristin Schwanke, Henning Kempf, Robert Zweigerdt, and Carl-Fredrik Mandenius

14. Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies

Sasitorn Rungarunlert, Joao N. Ferreira, and Andras Dinnyes

15. Whole Heart Construct Cultivation Under 3D Mechanical Stimulation of the Left Ventricle

Joern Hulsmann, Hug Aubin, Alexander Wehrmann, Alexander Jenke, Artur Lichtenberg, and Payam Akhyari

16. Tendon Differentiation on Decellularized Extracellular Matrix Under Cyclic Loading

Daniel W. Youngstrom and Jennifer G. Barrett

17. Bioengineered Models of Solid Human Tumors for Cancer Research

Alessandro Marturano-Kruik, Aranzazu Villasante, and Gordana Vunjak-Novakovic

18. Development of a Bladder Bioreactor for Tissue-Engineering in Urology

Niall F. Davis and Anthony Callanan

19. Use of Microfluidic Technology to Monitor the Differentiation and Migration of Human ESC-Derived Neural Cells

Jiwoo Bae, Nayeon Lee, Wankyu Choi, Suji Lee, Jung Jae Ko, Baek Soo Han, Sang Chul Lee, Noo Li Jeon, and Jihwan Song

Additional information

NLS9781493982134
9781493982134
1493982133
Bioreactors in Stem Cell Biology: Methods and Protocols by Kursad Turksen
New
Paperback
Humana Press Inc.
2018-05-30
238
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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