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Immobilization of Enzymes and Cells By Jose M. Guisan

Immobilization of Enzymes and Cells by Jose M. Guisan

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This book features step-by-step, readily reproducible laboratory protocols for the preparation, characterization, and utilization of immobilized enzymes and cells. It also features key notes and implementation advice from experts.

Immobilization of Enzymes and Cells Summary

Immobilization of Enzymes and Cells by Jose M. Guisan

For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

Table of Contents

1. New Opportunities for Immobilization of Enzymes

Jose M. Guisan

2. Immobilization of Enzymes: A Literature Survey Beatriz Brena, Paula Gonzalez-Pombo, and Francisco Batista-Viera

3. Glutaraldehyde Mediated Protein Immobilization

Fernando Lopez-Gallego, Jose M. Guisan,and Lorena Betancor

4. Immobilization of Enzymes on Mono-and Hetero-Functional Epoxy-Activated Supports

Cesar Mateo, Valeria Grazu, and Jose M. Guisan

5. Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Groups

Fernando Lopez-Gallego, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Cesar Mateo and Jose M. Guisan

6. Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports

Cesar Mateo, Gloria Fernandez-Lorente, Rocha-Martin, J., Bolivar, Juan, M., Jose M. Guisan

7. Reversible Covalent Immobilization of Enzymes Via Disulfide Bonds

Karen Ovsejevi , Carmen Manta, and Francisco Batista-Viera

8. Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in Low Water Media

Joyeeta Mukherjee, Kusum Solanki, and Munishwar Nath Gupta

9. Immobilization of Enzymes by Bioaffinity Layering

Veena Singh, Meryam Sardar, and Munishwar Nath Gupta

10. Immobilization of Enzymes on Magnetic Beads through Affinity Interactions

Audrey Sassolas, Akhtar Hayat and Jean-Louis Marty

11. Tips for the Functionalization of Nanoparticles with Antibodies

Ester Polo, Sara Puertas, Maria Moros, Pilar Batalla, Jose M. Guisan, Jesus M. de la Fuente, Valeria Grazu

12. Design and Characterization of Functional Nanoparticles for Enhanced Bio-Performance

Pablo del Pino, Scott G. Mitchell & Beatriz Pelaz

13. Immobilization of Enzymes on Ethynyl-modified Electrodes Via Click Chemistry

Akhtar Hayat, Audrey Sassolas, Amina Rhouati, Jean-Louis Marty

14. Modification of Carbon Nanotube Electrodes with 1-pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis

Guinevere Strack, Robert Nichols, Plamen Atanassov, Heather R. Luckarift, and Glenn R. Johnson

15. Enzyme Immobilization by Entrapment within a Gel Network

Audrey Sassolas, Akhtar Hayat and Jean-Louis Marty

16. Practical Protocols for Lipase Immobilization via Sol-Gel Techniques

Manfred T. Reetz

17. Improving Lipase Activity by Immobilization and Post-Immobilization Strategies

Jose M. Palomo, Marco Filice, Oscar Romero and Jose M. Guisan

18. High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic Solvents and Ionic Liquids

Ipsita Roy, Joyeeta Mukherjee, and Munishwar Nath Gupta

19. Biomedical Applications of Immobilized Enzymes: An Update

Marta Pastor, Amaia Esquisabel, and Jose Luis Pedraz

20. Immobilization of Whole Cells by Chemical Vapor Deposition of Silica

Susan R. Sizemore, Robert Nichols, Randi Tatum, Plamen Atanassov, Glenn R. Johnson, and Heather R. Luckarift

21. Encapsulation of Cells in Alginate Gels

Pello Sanchez, Rosa Maria Hernandez, Jose Luis Pedraz, and Gorka Orive

22. Microalgal Immobilization Methods

Ignacio Moreno-Garrido

23. Therapeutic Applications of Encapsulated Cells

Argia Acarregui, Gorka Orive, Jose Luis Pedraz, and Rosa Maria Hernandez

24. Whole Cell Entrapment Techniques

Jorge A. Trelles and Cintia W. Rivero

Additional information

Immobilization of Enzymes and Cells by Jose M. Guisan
Humana Press Inc.
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
This is a new book - be the first to read this copy. With untouched pages and a perfect binding, your brand new copy is ready to be opened for the first time

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