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Protein Expression in Mammalian Cells James L Hartley

Protein Expression in Mammalian Cells By James L Hartley

Protein Expression in Mammalian Cells by James L Hartley


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Protein Expression in Mammalian Cells Summary

Protein Expression in Mammalian Cells: Methods and Protocols by James L Hartley

Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.

Protein Expression in Mammalian Cells Reviews

From the reviews:

This book is really a great help for all those that wish to enter the field and I am sure even for those who are already involved with recombinant protein production in mammalian cells. ... the book is intended as well for basic researchers aimed to study native or recombinant proteins to highlight ... protein misfolding or the role of proteins in cancer and in general is intended for all those scientists aiming to contribute to solve the many mysteries still connoting the protein world. (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)

Table of Contents

1. Why Proteins in Mammalian Cells?

James L. Hartley

2. Large Scale Transfection of Mammalian Cells

Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm

3. Selection of High Expressing Mammalian Cells by Surface Display of Reporters

Christine T. DeMaria

4. Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles

Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash

5. Transfection of Difficult-to-Transfect Primary Mammalian Cells

Oliver Gresch and Ludger Altrogge

6. Stable Protein Expression in Mammalian Cells Using Baculoviruses

Andreas Lackner, Emanuel Kreidl, Barbara Peter-Voeroesmarty, Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch

7. Using Matrix Attachment Regions to Improve Recombinant Protein Production

Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, and Nicolas Mermod

8. Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells

Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, and Tina M. Sauerwald

9. Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells

Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang

10. Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells

Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, and Raymond J. Owens

11. Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker

Say Kong Ng

12. Tools for Co-Producing Multiple Proteins in Mammalian Cells

Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia

13. Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases

Paula Magnelli, Alicia Bielik, and Ellen Guthrie

14. Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA

Renate Kunert and Karola Vorauer-Uhl

15. Methods for Constructing Clones for Protein Expression in Mammalian Cells

Takefumi Sone and Fumio Imamoto

16. Optimizing Transient Recombinant Protein Expression in Mammalian Cells

Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito

Additional information

NLS9781493961603
9781493961603
1493961608
Protein Expression in Mammalian Cells: Methods and Protocols by James L Hartley
New
Paperback
Humana Press Inc.
2016-08-23
276
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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Customer Reviews - Protein Expression in Mammalian Cells