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DNA Nanotechnology By Giampaolo Zuccheri

DNA Nanotechnology by Giampaolo Zuccheri

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Nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the behavior and function of DNA. This book is a guide to scientists of all backgrounds and aims to ignite interest and activity in this young research field.

DNA Nanotechnology Summary

DNA Nanotechnology: Methods and Protocols by Giampaolo Zuccheri

Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Table of Contents

1. Synthesis and Characterization of Self-assembled DNA Nanostructures

Chenxiang Lin, Yonggang Ke, Rahul Chhabra, Jaswinder Sharma, Yan Liu, and Hao Yan

2. Protocols for Self-assembly and Imaging of DNA Nanostructures

Thomas L. Sobey and Friedrich C. Simmel

3. Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions

Hua Yang, Pik Kwan Lo, Christopher K. McLaughlin, Graham D. Hamblin, Faisal A. Aldaye, and Hanadi F. Sleiman

4. DNA-Templated Pd Conductive Metallic Nanowires

Khoa Nguyen, Stephane Campidelli, and Arianna Filoramo

5. A Method to Map Spatiotemporal pH Changes Inside Living Cells using a pH Triggered DNA Nanoswitch

Souvik Modi and Yamuna Krishnan

6. Control of Helical Handedness in DNA and PNA Nanostructures

Roberto Corradini, Tullia Tedeschi, Stefano Sforza, Mark M. Green, and Rosangela Marchelli

7. G-quartet, G-quadruplex, and G-wire Regulated by Chemical Stimuli

Daisuke Miyoshi and Naoki Sugimoto

8. Preparation and Atomic Force Microscopy of Quadruplex DNA

James Vesenka

9. Synthesis of Long DNA-based Nanowires

Alexander Kotlyar

10. G-wire Synthesis and Modification with Gold-nanoparticle

Christian Leiterer, Andrea Csaki, and Wolfgang Fritzsche

11. Preparation of DNA Nanostructures with Repetitive Binding Motifs by Rolling Circle Amplification

Edda Reiss, Ralph Hoelzel, and Frank F. Bier

12. Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-functionalization

Matteo Palma, Justin Abramson, Alon A. Gorodetsky, Colin Nuckolls, Michael P. Sheetz, Shalom J. Wind, and James Hone

13. Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces

Albert M. Hung and Jennifer N. Cha

14. DNA-modified Single Crystal and Nanoporous Silicon

Andrew Houlton, Bernard A. Connolly, Andrew R. Pike, and Benjamin R. Horrocks

15. The Atomic Force Microscopy as a Lithographic Tool: Nanografting of DNA Nanostructures for Biosensing Applications

Matteo Castronovo and Denis Scaini

16. Trapping and Immobilization of DNA Molecules Between Nanoelectrodes

Anton Kuzyk, J. Jussi Toppari, and Paivi Toerma

17. DNA Contour Length Measurements as a Tool for the Structural Analysis of DNA and Nucleoprotein Complexes

Claudio Rivetti

18. DNA Molecular Handles for Single Molecule Protein Folding Studies by Optical Tweezers

Ciro Cecconi, Elizabeth A. Shank, Susan Marqusee, and Carlos Bustamante

19. Optimal Practices for Surface-tethered Single Molecule Total Internal Reflection Fluorescence Resonance Energy Transfer Analysis

Matt V. Fagerburg and Sanford H. Leuba

20. Engineering Mononucleosomes for Single-Pair FRET Experiments

Wiepke J.A. Koopmans, Ruth Buning, and John van Noort

21. Measuring DNA-Protein Binding Affinity on a Single Molecule Using Optical Tweezers

Micah J. McCauley and Mark C. Williams

22. Modeling Nanopores for Sequencing DNA

Jeffrey R. Comer, David B. Wells, and Aleksei Aksimentiev

Additional information

DNA Nanotechnology: Methods and Protocols by Giampaolo Zuccheri
Humana Press Inc.
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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