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Microbial Proteomics By Doerte Becher

Microbial Proteomics by Doerte Becher

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Microbial Proteomics Summary

Microbial Proteomics: Methods and Protocols by Doerte Becher

This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond.
Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via

Table of Contents

Part I: Global Sample Preparation and Protein Enrichment

1. Filter-Aided Sample Preparation for Proteome Analysis

Jacek R. Wisniewski

2. Protein Enrichment from Highly Dilute Samples with StrataClean

Florian Bonn and Andreas Otto

Part II: Subcellular Fractionation

3. Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins

Susanne Sievers

4. Enrichment of Cell Surface-Associated Proteins in Gram+ Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis

Florian Bonn, Sandra Maass, and Jan Maarten van Dijl

5. Preparation of Bacterial Magnetosomes for Proteome Analysis

Oliver Raschdorf, Dirk Schuler, and Rene Uebe

6. Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics

Christian Manske, Ivo Finsel, Christine Hoffmann, and Hubert Hilbi

Part III: Protein Quantification

7. Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS

Martin Moche, Dirk Albrecht, Reiner Westermeier, and Knut Buttner

8. Applications of Difference Gel Electrophoresis (DIGE) on the Study of Microorganisms

Kathleen Trautwein and Ralf Rabus

9. Proteomic Signatures in Staphylococcus aureus

Susanne Engelmann and Stephan Fuchs

10. How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis

Alexander Reder, Stephan Michalik, and Ulf Gerth

11. Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE

Sandra Maass

12. Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses

Florian-Alexander Herbst, Nico Jehmlich, Martin von Bergen, and Frank Schmidt

13. Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)

Andreas Otto

14. Next-Generation Trapping of Protease Substrates by Label-Free Proteomics

Claudia Lindemann, Nikolas Thomanek, Katja Kuhlmann, Helmut E. Meyer, Katrin Marcus, and Franz Narberhaus

15. In Vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host-Pathogen Settings

Henrike Pfoertner, Maren Depke, Kristin Surmann, Frank Schmidt, and Uwe Voelker

Part IV: Analysis of Post Translational Protein Modifications

16. Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography

Boumediene Soufi, Christoph Taumer, Maja Semanjski, and Boris Macek

17. Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation

Angel Manteca, Beatriz Rioseras, Adelina Rogowska-Wrzesinska, and Ole N. Jensen

18. Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria

Martina Rossius, Falko Hochgrafe, and Haike Antelmann

Part V: Metaproteomics

19. Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host-Microbiota Interactions

Pranjul Shah, Emilie E.L. Muller, Laura A. Lebrun, Linda Wampach, and Paul Wilmes

20. Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis / Proteome Extraction from Soil Samples for Metaproteomics Studies

Karuna Chourey and Robert L. Hettich

21. Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter

Katharina M. Keiblinger and Katharina Riedel

22. Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria-Invertebrate Symbioses

Tjorven Hinzke, Manuel Kleiner, and Stephanie Markert

Additional information

Microbial Proteomics: Methods and Protocols by Doerte Becher
Humana Press Inc.
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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