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Plant Metabolomics By Carla Antonio

Plant Metabolomics by Carla Antonio

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Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.

Plant Metabolomics Summary

Plant Metabolomics: Methods and Protocols by Carla Antonio

This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.

Table of Contents

Part I: Review Chapters

1. The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments

Marina C.M. Martins, Camila Caldana, Lucia Daniela Wolf, and Luis Guilherme Furlan de Abreu

2. Standard Key Steps in Mass Spectrometry-Based Plant Metabolomics Experiments: Instrument Performance and Analytical Method Validation

Ana Margarida Rodrigues and Carla Antonio

3. Guidelines for Sample Normalization to Minimize Batch Variation for Large-Scale Metabolic Profiling of Plant Natural Genetic Variance

Saleh Alseekh, Si Wu, Yariv Brotman, and Alisdair R. Fernie

4. Gas Chromatography-Mass Spectrometry-Based 13C-Labeling Studies in Plant Metabolomics

Valeria F. Lima, Leonardo P. Souza, Thomas C.R. Williams, Alisdair R. Fernie, and Danilo M. Daloso

5. Carbon Atomic Survey for Identification of Selected Metabolic Fluxes

Leonardo Perez de Souza, Alisdair R. Fernie, and Takayuki Tohge

Part II: Mass Spectrometry-Based Methodology Chapters

6. Quantification of Low-Abundant Phosphorylated Carbohydrates Using HILIC-QqQ-MS/MS

Tiago F. Jorge and Carla Antonio

7. Quantification of Soluble Sugars and Sugar Alcohols by LC-MS/MS

Regina Feil and John Edward Lunn

8. Untargeted Analysis of Semi-Polar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation

Camille Benard, Sebastien Acket, Yannick Rossez, Olivier Fernandez, Thierry Berton, Yves Gibon, and Cecile Cabasson

9. Extraction of Plant Lipids for LC-MS-Based Untargeted Plant Lipidomics

Thusitha W.T. Rupasinghe and Ute Roessner

10. Semi-Targeted Lipidomics of Plant Acyl Lipids Using UPLC-HR-MS in Combination with a Data-Independent Acquisition Mode

Mohamed A. Salem and Patrick Giavalisco

11. Plant Lipidomics Using UPLC-QTOF-MS

Yozo Okazaki and Kazuki Saito

12. Targeted LC-MS Analysis for Plant Secondary Metabolites

Takafumi Shimizu, Mutsumi Watanabe, Alisdair R. Fernie, and Takayuki Tohge

13. An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones

Ondrej Novak and Kristyna Flokova

14. A Complete Pipeline for Generating a High-Resolution LC-MS-Based Reference Mass Spectra Library

Nir Shahaf, Asaph Aharoni, and Ilana Rogachev

15. Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS

Nirupama Samanmalie Jayasinghe, Himasha Mendis, Ute Roessner, and Daniel Anthony Dias

16. Acquisition of Volatile Compounds by Gas Chromatography-Mass Spectrometry (GC-MS)

Jose G. Vallarino, Alexander Erban, Ines Fehrle, Alisdair R. Fernie, Joachim Kopka, and Sonia Osorio

17. Mass Spectrometry Imaging (MSI) for Plant Metabolomics

Berin A. Boughton and Dinaiz Thinagaran

18. Laser Ablation Electrospray Ionization-Mass Spectrometry Imaging (LAESI-MS) for Spatially Resolved Plant Metabolomics

Desalegn Etalo, Carmen Diez-Simon, Ric C.H. de Vos, and Robert D. Hall

19. Live Single-Cell Mass Spectrometry (LSC-MS) for Plant Metabolomics

Keiko Masuda, Yasmine Abouleila, Ahmed Ali, Toshio Yanagida, and Tsutomu Masujima

Part III: Data Analysis & Statistics Chapters

20. Statistical and Multivariate Analysis of MS-Based Plant Metabolomics Data

Carsten Jaeger and Jan Lisec

21. Integration of Plant Metabolomics Data into Metabolic Networks: Progresses and Challenges

Nadine Toepfer, Samuel M.D. Seaver, and Asaph Aharoni

22. Understanding the Functionality of a Biological System as a Whole: Comparative Data Analysis

Isabel Orf

23. Regression-Based Modeling of Complex Plant Traits Based on Metabolomics Data

Francisco de Abreu e Lima, Lydia Leifels, and Zoran Nikoloski

24. Mathematical Modeling Approaches in Plant Metabolomics

Lisa Furtauer, Jakob Weiszmann, Wolfram Weckwerth, and Thomas Nagele

Additional information

Plant Metabolomics: Methods and Protocols by Carla Antonio
Humana Press Inc.
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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