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Peptide Self-Assembly By Bradley L. Nilsson

Peptide Self-Assembly by Bradley L. Nilsson

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Peptide Self-Assembly Summary

Peptide Self-Assembly: Methods and Protocols by Bradley L. Nilsson

This volume details methods and protocols on b-sheet assemblies and collagen. Divided into three parts chapters focus on expanding use of solid-state NMR as a powerful method to enhance structural understanding of self-assembled peptide materials, methods for the design, synthesis, and application of self-assembled peptide materials, and structural and mechanistic analyses of pathological amyloid systems that provide novel ways to assess function of the various possible aggregates as well to determine how the structure of these materials correlates to function/dysfunction in the biological context. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Peptide Self-Assembly: Methods and Protocols aims to capture modern methods that span the breadth of the exciting and expanding field of peptide self-assembly.


Table of Contents

Part I: Fundamental Methods for Characterizing Peptide Self-Assembly

1. Methods for Structural Analysis of Aggregates Formed by Self-Assembling Peptides

V. Castelletto and I. W. Hamley

2. Solid state NMR Structural Characterization of Self-assembled Peptides With Selective 13C and 15N Isotopic Labels

Danting Huang, Benjamin C. Hudson, Yuan Gao, Evan K. Roberts, and Anant K. Paravastu

3. ATR-FTIR Analysis of Amyloid Proteins

Jean-Marie Ruysschaert and Vincent Raussens

4. Imaging Protein Fibers at the Nanoscale and In Situ

Angelo Bella, Michael Shaw, Emiliana De Santis, and Maxim G. Ryadnov

5. Replica Exchange Molecular Dynamics: A Practical Application Protocol with Solutions to Common Problems and a Peptide Aggregation and Self-Assembly Example

Ruxi Qi, Guanghong Wei, Buyong Ma and Ruth Nussinov

6. An Aggregate Weight-normalized thioflavin-T Measurement Scale For Characterizing Polymorphic Amyloids And Assembly Intermediates

Ronald Wetzel, Saketh Chemuru, Pinaki Misra, Ravi Kodali, Smita Mukherjee, and Karunakar Kar

7. Nanoparticle Tracking for Protein Aggregation Research

Xiaomeng Lu and Regina M. Murphy

8. Peptide Self-assembly Measured using Fluorescence Correlation Spectroscopy

Judith J. Mittag, Joachim O. Radler, and Jennifer J. McManus

Part II: Methods for the Preparation of Functional Self-Assembled Peptide Biomaterials

9. A General Method to Prepare Peptide-based Supramolecular Hydrogels

Dan Yuan, Junfeng Shi, Ning Zhou, and Bing Xu

10. Recursive Directional Ligation Approach for Cloning Recombinant Spider Silks

Nina Dinjaski, Wenwen Huang, and David L. Kaplan

11. Synthesis of Mikto-Arm Star Peptide Conjugates

Jin Mo Koo, Hao Su, Yi-An Lin, and Honggang Cui

12. Synthesis and Evaluation of Self-Assembled Nanostructures of Peptide- chromophore conjugates

Tejaswini S. Kale and John D. Tovar

13. Programmable Fabrication of Multi-layer Collagen Nanosheets of Defined Composition

Tao Jiang and Vincent P. Conticello

14. Practical Considerations in the Design and use of Immunologically Active Fibrillar Peptide Assemblies

Carolina Mora Solano, Yi Wen, Huifang Han, and Joel H. Collier

15. Microwave-assisted Synthesis and Immunological Evaluation of Self-Assembling Peptide Vaccines

C. Brent Chesson, Rojelio Elias Alverado, and Jai S. Rudra

16. Preparation and Screening of Catalytic Amyloid Assemblies

Zsofia Lengyel, Caroline M. Rufo, and Ivan V. Korendovych

17. Self-assembly of Filamentous Cell Penetrating Peptides for Gene Delivery

Dawei Xu, Louis DeRidder, Brendan Elmore, and He Dong

18. Biogelx: Cell Culture on Gels based on Aromatic Peptide Amphiphiles

Mhairi M. Harper, Michael L. Connolly, Laura Goldie, Eleanore J. Irvine, Joshua E. Shaw, Vineetha Jayawarna, Stephen M. Richardson, Matthew J. Dalby, David Lightbody, and Rein V. Ulijn

Part III: Methods Related to Preparing, Characterizing and Inhibiting Pathological Amyloid Peptide Self-Assembly

19. Production and use of Recombinant A for Aggregation Studies

Tiernan T. O'Malley, Sara Linse, and Dominic M. Walsh

20. Disaggregation of Ab42 for Structural and Biochemical Studies

Hyewon Chung, Elliot Crooks, Martine Ziliox, and Steven O. Smith

21. Preparation of Stable Amyloid- Oligomers Without Perturbative Methods

Samuel A. Kotler and Ayyalusamy Ramamoorthy

22. Discriminating Strains of Self-Propagating Protein Aggregates Using a Conformational Stability Assay

Heather H.C. Lau, Angus Lau, and Joel C. Watts

23. Model Phospholipid Liposomes to Study the b-Amyloid-Peptide-Induced Membrane Disruption

Wei Qiang and Katelynne E. Doherty

24. Using Molecular Tweezers to Remodel Abnormal Protein Self-assembly and Inhibit the Toxicity of Amyloidogenic Proteins

Ravinder Malik, Jing Di, Gayatri Nair, Aida Attar, Karen Taylor, Edmond Teng, Frank-Gerrit Klarner, Thomas Schrader, and Gal Bitan

25. Incorporation of an azobenzene b-turn Peptidomimetic into Amyloid-b to Probe Potential Structural Motifs Leading to b-sheet self-assembly

Todd M. Doran and Bradley L. Nilsson

26. Solid State NMR Studies of Amyloid Materials: A Protocol to Define an Atomic Model of A (1-42) in Amyloid Fibrils

Yiling Xiao, Dan McElheny, Minako Hoshi, and Yoshitaka Ishii

27. Experimental and Computational Protocols for Studies of Cross-seeding Amyloid Assemblies

Baiping Ren, Rundong Hu, Mingzhen Zhang, Yonglan Liu, Lijian Xu, Binbo Jiang, Jie Ma, Buyong Ma, Ruth Nussinov, and Jie Zheng

Additional information

NPB9781493978090
9781493978090
1493978098
Peptide Self-Assembly: Methods and Protocols by Bradley L. Nilsson
New
Hardback
Humana Press Inc.
2018-05-10
452
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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